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Heat-Killed Lactobacillus brevis Enhances Phagocytic Activity and Generates Immune-Stimulatory Effects through Activating the TAK1 Pathway

Journal of Microbiology and Biotechnology 2020년 30권 9호 p.1395 ~ 1403
정민주, 김재환, 이지수, 강신달, 심상민, 정문영, 양희, 변상균, 이기원,
소속 상세정보
정민주 ( Jeong Min-Ju ) - Seoul National University Department of Agricultural Biotechnology
김재환 ( Kim Jae-Hwan ) - Seoul National University Department of Agricultural Biotechnology
이지수 ( Lee Ji-Su ) - Incheon National University Division of Bioengineering
강신달 ( Kang Shin-Dal ) - SPC Group Research Institute of Food and Biotechnology
심상민 ( Shim Sang-Min ) - SPC Group Research Institute of Food and Biotechnology
정문영 ( Jung Moon-Young ) - SPC Group Research Institute of Food and Biotechnology
양희 ( Yang Hee ) - Seoul National University Center for Food and Bioconvergence
변상균 ( Byun Sang-Uine ) - Yonsei University Department of Biotechnology
이기원 ( Lee Ki-Won ) - Seoul National University Department of Agricultural Biotechnology

Abstract


There is an increasing interest in using inactivated probiotics to modulate the host immune system and protect against pathogens. As the immunomodulatory function of heat-killed Lactobacillus brevis KCTC 12777BP (LBB) and its mechanism is unclear, we investigated the effect of LBB on immune response based on the hypothesis that LBB might exert stimulatory effects on immunity. In the current study, we demonstrate that administration of LBB can exert immune-stimulatory effects and promote clearance of foreign matters through enhancing phagocytosis. Treatment with LBB induced the production of TNF-α, IL-6, and nitric oxide in macrophages. Importantly, LBB directly increased the phagocytic activity of macrophages against bacterial particles. LBB was able to promote the production of TNF-α in bone marrow-derived macrophages and splenocytes and also increase the proliferation rate of splenocytes, suggesting that the immune-stimulating activity of LBB can be observed in primary immune cells. Investigation into the molecular mechanism responsible revealed that LBB upregulates TAK1 activity and its downstream ERK, p38, and JNK signaling pathways. To further confirm the immunomodulatory capability of LBB in vivo, we orally administered LBB to mice and assessed the effect on primary splenocytes. Splenocytes isolated from LBB-treated mice exhibited higher TNF-α expression and proliferative capacity. These results show that heat-killed L. brevis, a wildly consumed probiotic, may provide protection against pathogens through enhancing host immunity.

키워드

Lactobacillus brevis; probiotics; immune stimulation; TAK1; phagocytosis

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