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1-Methoxylespeflorin G11 Protects HT22 Cells from Glutamate-Induced Cell Death through Inhibition of ROS Production and Apoptosis

Journal of Microbiology and Biotechnology 2021년 31권 2호 p.217 ~ 225
이필준, Pham Chau Ha, Thuy Nguyen Thi Thanh, 박혜진, 이성훈, 유희민, 조남기,
소속 상세정보
이필준 ( Lee Phil-Jun ) - Ajou University College of Pharmacy
 ( Pham Chau Ha ) - Korea Research Institute of Standards and Science Biometrology Group
 ( Thuy Nguyen Thi Thanh ) - Chonnam National University College of Pharmacy
박혜진 ( Park Hye-Jin ) - Ajou University College of Pharmacy
이성훈 ( Lee Sung-Hoon ) - Chung-Ang University College of Pharmacy
유희민 ( Yoo Hee-Min ) - Korea Research Institute of Standards and Science Biometrology Group
조남기 ( Cho Nam-Ki ) - Chonnam National University College of Pharmacy

Abstract


This study aimed to investigate the neuroprotective effects of 1-methoxylespeflorin G11 (MLG), a pterocarpan, against glutamate-induced neurotoxicity in neuronal HT22 hippocampal cells. The protective effects of MLG were evaluated using MTT assay and microscopic analysis. The extent of apoptosis was studied using flow cytometric analysis performed on the damaged cells probed with annexin V/propidium iodide. Moreover, mitochondrial reactive oxygen species (ROS) were assessed using flow cytometry through MitoSOXTM Red staining. To determine mitochondrial membrane potential, staining with tetramethylrhodamine and JC-1 was performed followed by flow cytometry. The results demonstrated that MLG attenuates glutamate-induced apoptosis in HT22 cells by inhibiting intracellular ROS generation and mitochondrial dysfunction. Additionally, MLG prevented glutamate-induced apoptotic pathway in HT22 cells through upregulation of Bcl-2 and downregulation of cleaved PARP-1, AIF, and phosphorylated MAPK cascades. In addition, MLG treatment induced HO-1 expression in HT22 cells. These results suggested that MLG exhibits neuroprotective effects against glutamate-induced neurotoxicity in neuronal HT22 cells by inhibiting oxidative stress and apoptosis.

키워드

1-Methoxylespeflorin G11; HT22 cells; reactive oxygen species (ROS); apoptosis; mitochondrial membrane potential (MMP)

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