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Production of Algal Biomass and High-Value Compounds Mediated by Interaction of Microalgal Oocystis sp. KNUA044 and Bacterium Sphingomonas KNU100

Journal of Microbiology and Biotechnology 2021년 31권 3호 p.387 ~ 397
나호, 조승우, 도정미, 김일섭, 윤호성,
소속 상세정보
나호 ( Na Ho ) - Kyungpook National University Department of Biology
조승우 ( Jo Seung-Woo ) - Kyungpook National University Advanced Bio-resource Research Center
도정미 ( Do Jeong-Mi ) - Kyungpook National University Department of Biology
김일섭 ( Kim Il-Sup ) - Kyungpook National University Advanced Bio-resource Research Center
윤호성 ( Yoon Ho-Sung ) - Kyungpook National University Department of Biology

Abstract


There is growing interest in the production of microalgae-based, high-value by-products as an emerging green biotechnology. However, a cultivation platform for Oocystis sp. has yet to be established. We therefore examined the effects of bacterial culture additions on the growth and production of valuable compounds of the microalgal strain Oocystis sp. KNUA044, isolated from a locally adapted region in Korea. The strain grew only in the presence of a clear supernatant of Sphingomonas sp. KNU100 culture solution and generated 28.57 mg/l/d of biomass productivity. Protein content (43.9 wt%) was approximately two-fold higher than carbohydrate content (29.4 wt%) and lipid content (13.9 wt%). Oocystis sp. KNUA044 produced the monosaccharide fucose (33 μg/mg and 0.94 mg/l/d), reported here for the first time. Fatty acid profiling showed high accumulation (over 60%) of polyunsaturated fatty acids (PUFAs) compared to saturated (29.4%) and monounsaturated fatty acids (9.9%) under the same culture conditions. Of these PUFAs, the algal strain produced the highest concentration of linolenic acid (C18:3 ω3; 40.2%) in the omega?3 family and generated eicosapentaenoic acid (C20:5 ω3; 6.0%), also known as EPA. Based on these results, we suggest that the application of Sphingomonas sp. KNU100 for strain-dependent cultivation of Oocystis sp. KNUA044 holds future promise as a bioprocess capable of increasing algal biomass and high-value bioactive by-products, including fucose and PUFAs such as linolenic acid and EPA.

키워드

Oocystis spp.; Sphingomonas spp.; symbiotic interaction; algal biomass; eicosapentaenoic acid; fucose

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