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Genetic Background Behind the Amino Acid Profiles of Fermented Soybeans Produced by Four Bacillus spp.

Journal of Microbiology and Biotechnology 2021년 31권 3호 p.447 ~ 455
장미현, 정도원, 허강훈, 공하람, 김청태, 이종훈,
소속 상세정보
장미현 ( Jang Mi-Hyun ) - Kyonggi University Department of Food Science and Biotechnology
정도원 ( Jeong Do-Won ) - Dongduk Women’s University Department of Food and Nutrition
허강훈 ( Heo Gang-Hun ) - Kyonggi University Department of Food Science and Biotechnology
공하람 ( Kong Ha-Ram ) - Kyonggi University Department of Food Science and Biotechnology
김청태 ( Kim Cheong-Tae ) - Nongshim Co., Ltd.
이종훈 ( Lee Jong-Hoon ) - Kyonggi University Department of Food Science and Biotechnology

Abstract


Strains of four Bacillus spp. were respectively inoculated into sterilized soybeans and the free amino acid profiles of the resulting cultures were analyzed to discern their metabolic traits. After 30 days of culture, B. licheniformis showed the highest production of serine, threonine, and glutamic acid; B. subtilis exhibited the highest production of alanine, asparagine, glycine, leucine, proline, tryptophan, and lysine. B. velezensis increased the γ-aminobutyric acid (GABA) concentration to >200% of that in the control samples. B. sonorensis produced a somewhat similar amino acid profile with B. licheniformis. Comparative genomic analysis of the four Bacillus strains and the genetic profiles of the produced free amino acids revealed that genes involved in glutamate and arginine metabolism were not common to the four strains. The genes gadA/B (encoding a glutamate decarboxylase), rocE (amino acid permease), and puuD (γ-glutamyl-γ-aminobutyrate hydrolase) determined GABA production, and their presence was species-specific. Taken together, B. licheniformis and B. velezensis were respectively shown to have high potential to increase concentrations of glutamic acid and GABA, while B. subtilis has the ability to increase essential amino acid concentrations in fermented soybean foods.

키워드

Soybean fermentation; Bacillus; γ-aminobutyric acid; gadA/B; rocE; puuD

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