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Improvement of Unsaturated Fatty Acid Production from Porphyridium cruentum Using a Two-Phase Culture System in a Photobioreactor with Light-Emitting Diodes (LEDs)

Journal of Microbiology and Biotechnology 2021년 31권 3호 p.456 ~ 463
김소희, 이의훈, 이상백, 정귀택, 김성구,
소속 상세정보
김소희 ( Kim So-Hee ) - Pukyong National University School of Marine, Fisheries and Life Science
이의훈 ( Lee Ui-Hun ) - Pukyong National University School of Marine, Fisheries and Life Science
이상백 ( Lee Sang-Baek ) - Pukyong National University School of Marine, Fisheries and Life Science
정귀택 ( Jeong Gwi-Taek ) - Pukyong National University School of Marine, Fisheries and Life Science
김성구 ( Kim Sung-Koo ) - Pukyong National University School of Marine, Fisheries and Life Science

Abstract


In this study, the culture conditions for Porphyridium cruentum were optimized to obtain the maximum biomass and lipid productions. The eicosapentaenoic acid content was increased by pH optimization. P. cruentum was cultured with modified F/2 medium in 14-L photobioreactors using a two-phase culture system, in which the green (520 nm) and red (625 nm) light-emitting diodes (LEDs) were used during the first and second phases for biomass production and lipid production, respectively. Various parameters, including aeration rate, light intensity, photoperiod, and pH were optimized. The maximum biomass concentration of 0.91 g dcw/l was obtained with an aeration rate of 0.75 vvm, a light intensity of 300 μmol m-2s-1, and a photoperiod of 24:0 h. The maximum lipid production of 51.8% (w/w) was obtained with a light intensity of 400 μmol m-2s-1 and a photoperiod of 18:6 h. Additionally, the eicosapentaenoic acid and unsaturated fatty acid contents reached 30.6% to 56.2% at pH 6.0.

키워드

Light-emitting diodes (LEDs); unsaturated fatty acids; photobioreactor; Porphyridium cruentum; eicosapentaenoic acid

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