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Fine Mapping and Candidate Gene Analysis of the Floury Endosperm Gene, FLO(a), in Rice

Molecules and Cells 2010년 29권 2호 p.167 ~ 174
Yongli Qiao, 이송이, Rihua Piao, Jiang Wenzhu, Ham Tae-Ho, 진중현, Piao Zhongze, Han Longzhi, 강시용, 고희종,
소속 상세정보
 ( Yongli Qiao ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science
이송이 ( Lee Song-I ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science
 ( Rihua Piao ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science
 ( Jiang Wenzhu ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science
 ( Ham Tae-Ho ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science
진중현 ( Chin Joong-Hyoun ) - International Rice Research Institute Genetics and Biotechnology Plant Breeding
 ( Piao Zhongze ) - China Shanghai Academy of Agricultural Sciences Crop Research Institute
 ( Han Longzhi ) - Chinese Academy of Agricultural Sciences Ministry of Agriculture, Institute of Crop Science Key Laboratory of Crop Germplasm Resources and Biotechnology
강시용 ( Kang Si-Yong ) - Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
고희종 ( Koh Hee-Jong ) - Seoul National University and Plant Genomics and Breeding Institute Research Institute for Agriculture and Life Sciences Department of Plant Science

Abstract


In addition to its role as an energy source for plants, ani-mals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been ex-plored as an agronomical trait in breeding and genetics studies. In the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of Oryza sativa ssp. japonica cultivar Hwacheong with MNU. The innermost endosperm of the flo(a) mutant exhibited floury characteristics while the outer layer of the endosperm appeared normal. Starch granules in the flo(a) mutant formed a loosely-packed crystalline structure and X-ray diffraction revealed that the overall crystallinity of the starch was decreased compared to wild-type. The FLO(a) gene was isolated via a map-based cloning approach and predicted to encode the tetratricopeptide repeat domain-containing protein, OsTPR. Three mutant alleles contain a nucleotide substitution that generated one stop codon or one splice site, respectively, which presumably disrupts the interaction of the functionally conserved TPR motifs. Taken together, our map-based cloning approach pin-pointed an OsTPR as a strong candidate of FLO(a), and the proteins that contain TPR motifs might play a significant role in rice starch biosynthetic pathways.

키워드

endosperm; mapping; rice; starch; tetratricopeptide repeat domain-containing protein

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