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Tsg101 Is Necessary for the Establishment and Maintenance of Mouse Retinal Pigment Epithelial Cell Polarity

Molecules and Cells 2021년 44권 3호 p.168 ~ 178
Le Dai, 임소연, 민광욱, 박준우, 김유정, 하태정, Moon Kyeong-Hwan, Wagner Kay-Uwe, 김진우,
소속 상세정보
 ( Le Dai ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
임소연 ( Lim So-Yeon ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
민광욱 ( Min Kwang-Wook ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
박준우 ( Park Joon-Woo ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
김유정 ( Kim You-Joung ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
하태정 ( Ha Tae-Jeong ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
 ( Moon Kyeong-Hwan ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
 ( Wagner Kay-Uwe ) - Wayne State University Department of Oncology
김진우 ( Kim Jin-Woo ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences

Abstract


The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.

키워드

cell polarity; endosomal sorting complexes required for transport (ESCRT); retinal pigment epithelium (RPE); tumor susceptibility gene 101 (Tsg101)

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