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흰쥐 혈액-태반 관문의 태아측 합포체성 영양세포막에서 콜린의 수송

Transport of the choline at rat syncytiotrophoblast cell lines, TR-TBT 18d-2 through the blood-placental barrier

약학논문집-숙명대학교 2006년 23권 1호 p.13 ~ 17
최홍미, 강영숙,
소속 상세정보
최홍미 ( Choi Hong-Mi ) - 숙명여자대학교 약학대학
강영숙 ( Kang Young-Sook ) - 숙명여자대학교 약학대학

Abstract


The placental transport of choline is very important because the fetus cannot supply it by themselves and it is required for methylation, biosynthesis phospholipids and cell signaling. We examined the characteristics of choline transport across the placenta using rat conditionally immortalized syncytiotrophoblast cell lines ( TR-TBT) in vitro. The uptake of [3H]choline in TR-TBT 18d-2 cells was increased with increasing time. The choline transport process was saturable with a Michalis-Menten constant (Km) of 130 ± 37 μM, a Kd of 0.826 ± 0.084 μM and a Vmax of 826 ± 223 pmol/mg protein/min in TR-TBT 18d-2 cells. [3H]Choline uptake was significantly inhibited by an excess amount of choline itself and by hemicholinium-3, a structural analog of choline. However organic anion, nicotinic acid, did not affect [3H]choline uptake in TR-TBT 18d-2 cells. In addition, [3H]choline uptake in this cells, blood-placental barrier, was Na+-and pH-dependent. These results suggest that choline may be transported by a carrier-mediated transport system in TR-TBT 18d-2 cells.

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