조골세포에 있어서 형질전환 효율의 최적화
Optimization of Transfection Efficiency in Osteoblastic Cells
약학논문집-숙명대학교 2006년 23권 1호 p.55 ~ 58
전효정, 김숙현, 양희정, 박효정, 임미정,
소속 상세정보
전효정 ( Jun Hyo-Jung ) - 숙명여자대학교 약학대학
김숙현 ( Kim Sook-Hyun ) - 숙명여자대학교 약학대학
양희정 ( Yang Hee-Jung ) - 숙명여자대학교 약학대학
박효정 ( Park Hyo-Jung ) - 숙명여자대학교 약학대학
임미정 ( Yim Mi-Jung ) - 숙명여자대학교 약학대학
KMID : 0606320060230010055
Abstract
Achievement of optimal transfection efficiency is invaluable for mechanistic studies on osteoblastic gene regulation. In the present study, we have shown that the efficiency of biochemical transfection reagents varies significantly in UAMS-32 osteoblastic cells. The highest transfection efficiency was obtained when 4 mg DNA and Welfect-plus ™was used in UAMS-32 osteoblastic cells. We also showed the evidence of the applicability of this method for the efficient transfection of osteoblastic cells with reporter constructs for luciferase reporter assay.
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