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The Heterotrimeric Kinesin-2 Family Member KIF3A Directly Binds to Creatine Kinase B

Journal of Life Science 2021년 31권 3호 p.257 ~ 265
정영주, 박성우, 서미경, 김상진, 이원희, 김무성, 엄상화, 이정구, 석대현,
소속 상세정보
정영주 ( Jeong Young-Joo ) - Inje University College of Medicine Department of Biochemistry
박성우 ( Park Sung-Woo ) - Inje University College of Medicine Department of Convergence Biomedical Science
서미경 ( Seo Mi-Kyoung ) - Inje University Paik Institute for Clinical Research
김상진 ( Kim Sang-Jin ) - Inje University College of Medicine Department of Neurology
이원희 ( Lee Won-Hee ) - Inje University College of Medicine Department of Neurosurgery
김무성 ( Kim Moo-Seong ) - Inje University College of Medicine Department of Neurosurgery
엄상화 ( Urm Sang-Hwa ) - Inje University College of Medicine Department of Preventive Medicine
이정구 ( Lee Jung-Goo ) - Inje University College of Medicine Haeundae Paik Hospital Department of Psychiatry
석대현 ( Seog Dae-Hyun ) - Inje University College of Medicine Department of Biochemistry

Abstract


Heterotrimeric kinesin-2 is a molecular motor protein of the kinesin superfamily (KIF) that moves along a microtubule plus-end directed motor protein. It consists of three different motor subunits (KIF3A, KIF3B, and KIF3C) and a kinesin-associated protein 3 (KAP3) that form a heterotrimeric complex. Heterotrimeric kinesin-2 interacts with many different binding proteins through the cargo-binding domain of the KIF3s. The activity of heterotrimeric kinesin-2 is regulated to ensure that the cargo is directed to the right place at the right time. How this regulation occurs, however, remains in question. To identify the regulatory proteins for heterotrimeric kinesin-2, we performed yeast two-hybrid screening and found a specific interaction with creatine kinase B (CKB), which is the brain isoform of cytosolic creatine kinase enzyme. CKB bound to the cargo-binding domain of KIF3A but did not interact with the KIF3B, KIF5B, or KAP3 in the yeast two-hybrid assay. The carboxyl (C)-terminal region of CKB is essential for the interaction with KIF3A. Another protein kinase, CaMKIIa, interacted with KIF3A, but GSK3a did not interact with KIF3A in the yeast two-hybrid assay. KIF3A interacted with GST-CKB-C but not with GSK-CKB-N or GST alone. When co-expressed in HEK-293T cells, CKB co-localized with KIF3A and co-immunoprecipitated with KIF3A and KIF3B but not KIF5B. These results suggest that the CKB-KIF3A interaction may regulate the cargo transport of heterotrimeric kinesin-2 under energy-compromised conditions in cells.

키워드

Binding protein; creatine kinase; kinesin; phosphorylation

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