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자소엽에서 분리된 트리테르페노이드의 베타-아밀로이드 응집 억제 효과

Anti-Amyloidogenic Effects of Triterpenoids Isolated from Perilla Leaves

생약학회지 2020년 51권 4호 p.238 ~ 243
여지윤, 이충현, 박소영,
소속 상세정보
여지윤 ( Yeo Ji-Yun ) - Dankook University College of Pharmacy
이충현 ( Lee Choong-Hyun ) - Dankook University College of Pharmacy
박소영 ( Park So-Young ) - Dankook University College of Pharmacy

Abstract


Perilla frutescens Britton var. acuta Kudo, an annual plant primarily cultivated in China, Japan, and Korea, has been used as a traditional medicine to treat inflammatory diseases, depression, and many anxiety-related disorders. Previously, we reported the inhibitory effects of n-hexane layer of P. frutescens var. acuta extract against beta-amyloid (Aβ) aggregation, and the isolation of asarone derivatives as active constituents from n-hexane layer. In this study, dichloromethane layer of P. frutescens var. acuta was applied to bioassay-guided isolation methods accompanied with Thioflavin T (Th T) fluorescence assay to investigate the inhibitory effect on Aβ aggregation and disaggregation. As the results, three triterpenoids including ursolic acid (1), tormentic acid (2) and corosolic acid (3) were isolated. All compounds reduced Aβ aggregation and increased disaggregation of preformed Aβ aggregates in a dose-dependent manner. However, the inhibitory effect of three compounds on Aβ aggregation was not correlated with antioxidant activity, which was measured by DPPH assay. Taken together, these results suggest that the triterpenoid derivatives from P. frutescens have the potential to be developed as good therapeutics or preventatives for AD.

키워드

Perilla frutescens var. acuta; Beta-amyloid; Aggregation; Disaggregation; Triterpenoids

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