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백출에서 분리된 Atractylenolide II의 RAW264.7 대식세포와 BV2 미세아교세포에서의 항염증 효과

Atractylenoide II Isolated from Atractylodes macrocephala Inhibited Inflammatory Responses in Lipopolysaccharide-induced RAW264.7 Macrophages and BV2 Microglial Cells

생약학회지 2020년 51권 4호 p.244 ~ 254
김홍광, 김관우, 이정, 임혜리, 이대영, 윤다혜, 정진태, 김금숙, 오현철, 안인파, 김윤철,
소속 상세정보
김홍광 ( Kim Hong-Guang ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development
김관우 ( Kim Kwan-Woo ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development
이정 ( Li Jing ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development
임혜리 ( Im Hye-Ri ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development
이대영 ( Lee Dae-Young ) - RDA National Institute of Horticultural and Herbal Science Department of Herbal Crop Research
윤다혜 ( Yoon Da-Hye ) - RDA National Institute of Horticultural and Herbal Science Department of Herbal Crop Research
정진태 ( Jeong Jin-Tae ) - RDA National Institute of Horticultural and Herbal Science Department of Herbal Crop Research
김금숙 ( Kim Geum-Soog ) - RDA National Institute of Horticultural and Herbal Science Department of Herbal Crop Research
오현철 ( Oh Hyun-Cheol ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development
안인파 ( An Ren-Bo ) - Yanbian University College of Pharmacy
김윤철 ( Kim Yoon-Chul ) - Wonkwang University College of Pharmacy Institute of Pharmaceutical Research and Development

Abstract


Atractylodes macrocephala is a perennial herb and is a member of the Compositae family. This plant is known to contain various bioactive constituents indicating anti-inflammatory, neuroprotective, anti-oxidant, immunological enhancement, and gastroprotective effects. In this investigation, we isolated four compounds with similar chemical structures from A. macrocephala, and evaluated their anti-inflammatory effects. Among the four compounds, compound 2(atractylenolide II) showed the second-best inhibitory effect on the lipopolysaccharide(LPS)-induced production of nitric oxide in RAW264.7 macrophages and BV2 microglial cells. Compound 2 also inhibited the LPS-induced the production of prostaglandin E2(PGE2), and the expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2 proteins in both cells. In addition, compound 2 suppressed the production of pro-inflammatory cytokines including interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-a. These inhibitory effects were contributed by inactivation of nuclear factor kappa B(NF-κB) and mitogen-activated protein kinases(MAPKs) pathways by treatment with compound 2. This compound did not induce the expression of heme oxygenase(HO)-1 protein indicating that the anti-inflammatory effect of compound 2 was independent with HO-1 protein. Taken together, these results suggested that atractylenolide II can be a candidate material to treat inflammatory diseases.

키워드

Atractylodes macrocephala; Atractylenolide; Anti-inflammation; Nuclear factor kappa B; Mitogen-activated protein kinases

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