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청피 물 추출물의 항염증 효과와 기전 연구

Anti-inflammatory Effect and Mechanism of Citri Reticulatae Viride Pericarpium Water Extract

대한한방부인과학회지 2021년 34권 1호 p.34 ~ 47
안태석, 황덕상, 이진무, 장준복, 이창훈,
소속 상세정보
안태석 ( Ahn Tae-Seok ) - Kyung Hee University Graduate School Department of Clinical Korean Medicine
황덕상 ( Hwang Deok-Sang ) - Kyung Hee University College of Korean Medicine Department of Gynecology
이진무 ( Lee Jin-Moo ) - Kyung Hee University College of Korean Medicine Department of Gynecology
장준복 ( Jang Jun-Bock ) - Kyung Hee University College of Korean Medicine Department of Gynecology
이창훈 ( Lee Chang-Hoon ) - Kyung Hee University College of Korean Medicine Department of Gynecology

Abstract


Objective: This study was designed to examine anti-inflammatory effect and mechanism of Citri Reticulatae Viride Pericarpium water extract (CRE).

Methods: Cell cytotoxicity was tested with RAW 264.7 cells. To investigate anti-inflammatory effect of CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). In addition, mitrogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) were examined by western blotting in LPS-induced RAW 264.7 cell with treated CRE.

Results: In cytotoxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a result of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway.

Conclusion: CRE might have anti-inflammatory effect in LPS-induced macrophages by promoting the production of IL-10.

키워드

Citri Reticulatae Viride Pericarpium; Anti-inflammatory; Lipopolysaccharide; Macrophage; Interleukin-10

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