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오수유의 메탄올 추출 농도에 따른 항산화와 AGS세포에 대한 독성 효과

Effect of Methanol Extract Concentration on the Anti-oxidative Activity and Toxicity of Evodiae Fructus to AGS Cells

한국식생활문화학회지 2020년 35권 4호 p.400 ~ 405
양지영, 변휘용, 김진우, 김사현, 이평재,
소속 상세정보
양지영 ( Yang Ji-Yeong ) - Yonsei University Wonju College of Health Sciences Department of Biomedical Laboratory Science
변휘용 ( Byeon Hwi-Yong ) - Semyung University School of Oriental Medicine and Bio Convergence Sciences
김진우 ( Kim Jin-Woo ) - Semyung University School of Oriental Medicine and Bio Convergence Sciences
김사현 ( Kim Sa-Hyun ) - Semyung University Department of Clinical Laboratory Science
이평재 ( Lee Pyeong-Jae ) - Semyung University School of Industrial Bio-Pharmaceutical Science

Abstract


Evodiae Fructus is the dried unripe fruit of Evodia rutaecarpa, and has traditionally been used for treating stomachacheand diarrhea. Evodiamine and rutaecarpine, the major biologically active compounds of Evodiae Fructus, are reported tohave anti-oxidative and anti-inflammatory effects, as well as inhibit proliferation and metastasis of various cancer cells. Thecurrent study investigates the anti-oxidative and anti-cancer effects of the Evodiae Fructus extract, considering varyingconcentrations of methanol extraction (40, 80, and 95%). High contents of total phenolic compounds were determined inthe order of extracts 80, 95, and 40%. Evaluating contents of the 95, 80, and 40% extracts revealed 36.77, 7.29, and 1.86μg/mg evodiamine, respectively, and 53.02, 17.16, and 3.79 μg/mg rutaecarpine, respectively, with the highest content ofboth compounds obtained in the 95% extract. DPPH radical scavenging activity was observed to be inversely proportionalto the contents of total phenolic compounds, with decreasing SC50 values obtained in the order 80, 95, and 40% extract. The 95 and 80% extracts exerted toxicity to AGS gastric cancer cells, but the 40% extract was non-toxic. Evodiamine is aknown anti-cancer agent, and could be responsible for the observed toxicity. Cleavage of PARP, and Caspase-3, -7, -8 and-9 was observed in the 95% extract-treated AGS cells, indicating that cell toxicity exerted by the 95% extract could beattributed to apoptosis.

키워드

Evodia rutaecarpa; evodiamine; rutaecarpine;anti-oxidative effect; gastric cancer cell

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