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Alpha-lipoic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced cell injury by inhibiting autophagy and apoptosis

International Journal of Oral Biology 2021년 46권 1호 p.15 ~ 22
강경록, 김재성, 김태현, 서정연, 임향이, 박종현, 양광열, 유선경, 김흥중, 김춘성, 전홍성, 이동설, 박주철, 김도경,
소속 상세정보
강경록 ( Kang Kyeong-Rok ) - Chosun University Institute of Dental Science
김재성 ( Kim Jae-Sung ) - Chosun University Institute of Dental Science
김태현 ( Kim Tae-Hyeon ) - Chosun University Institute of Dental Science
서정연 ( Seo Jeong-Yeon ) - Chosun University Department of Integrative Biological Sciences
임향이 ( Lim Hyang-I ) - Chosun University Institute of Dental Science
박종현 ( Park Jong-Hyun ) - Chosun University Institute of Dental Science
양광열 ( Yang Kwang-Yeol ) - Chosun University Institute of Dental Science
유선경 ( Yu Sun-Kyoung ) - Chosun University Institute of Dental Science
김흥중 ( Kim Heung-Joong ) - Chosun University Institute of Dental Science
김춘성 ( Kim Chun-Sung ) - Chosun University Institute of Dental Science
전홍성 ( Chun Hong-Sung ) - Chosun University Department of Integrative Biological Sciences
이동설 ( Lee Dong-Seol ) - Seoul National University School of Dentistry Department of Oral Histology-Developmental Biology
박주철 ( Park Joo-Cheol ) - Seoul National University School of Dentistry Department of Oral Histology-Developmental Biology
김도경 ( Kim Do-Kyung ) - Chosun University Institute of Dental Science

Abstract


Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and BclxL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.

키워드

Alpha-lipoic acid; Autophagy; Hydrogen peroxide; Neuronal cell

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