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Cypress Essential Oil Improves Scopolamine-induced Learning and Memory Deficit in C57BL/6 mice

대한본초학회지 2020년 35권 5호 p.33 ~ 39
이길용, 이찬, 백정인, 배근영, 박찬익, 장정희,
소속 상세정보
이길용 ( Lee Gil-Yong ) - Daegu Hanny University Department of Aroma-Applied Industry
이찬 ( Lee Chan ) - Keimyung University School of Medicine Department of Pharmacology
백정인 ( Baek Jeong-In ) - Daegu Hanny University Department of Aroma-Applied Industry
배근영 ( Bae Keun-Young ) - University of Pennsylvania Roy and Diana Vagelos Program in Life Sciences and Management
박찬익 ( Park Chan-Ik ) - Daegu Hanny University Department of Aroma-Applied Industry
장정희 ( Jang Jung-Hee ) - Keimyung University School of Medicine Department of Pharmacology

Abstract


Objectives : Increasing evidence supports the biological and pharmacological activities of essential oils on the central nervous system such as pain, anxiety, attention, arousal, relaxation, sedation and learning and memory. The purpose of present work is to investigate the protective effect and molecular mechanism of cypress essential oil (CEO) against scopolamine (SCO)-induced cognitive impairments in C57BL/6 mice.

Methods : A series of behavior tests such as Morris water maze, passive avoidance, and fear conditioning tests were conducted to monitor learning and memory functions. Immunoblotting and RT-PCR were also performed in the hippocampal tissue to determine the underlying mechanism of CEO.

Results : SCO induced cognitive impairments as assessed by decreased step-through latency in passive avoidance test, relatively low freezing time in fear conditioning test, and increased time spent to find the hidden platform in Morris water maze test. Conversely, CEO inhalation significantly reversed the SCO-induced cognitive impairments in C57BL/6 mice comparable to control levels. To elucidate the molecular mechanisms of memory enhancing effect of CEO we have examined the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus. CEO effectively elevated the protein as well as mRNA expression of BDNF via activation of cAMP response element binding protein (CREB).
Conclusions : Our findings suggest that CEO inhalation effectively restored the SCO-impaired cognitive functions in C56BL/6 mice. This learning and memory enhancing effect of CEO was partly mediated by up-regulation of BDNF via activation of CREB.

키워드

brain-derived neurotrophic factor; cAMP response element binding protein; cypress essential oil; learning and memory impairment;scopolamine

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