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육군자탕의 정제 개발과 성분함량 및 약리효과 평가

Development of tablets and evaluation of ingredient content and pharmacological effects of Yukgunja-tang

대한본초학회지 2021년 36권 1호 p.67 ~ 76
김명진, 최혜민, 유병우,
소속 상세정보
김명진 ( Kim Myoung-Jin ) - National Institute for Korean Medicine Development
최혜민 ( Choi Hye-Min ) - National Institute for Korean Medicine Development
유병우 ( Yu Byung-Woo ) - National Institute for Korean Medicine Development

Abstract


Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development.

Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 ㎎/㎏) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment.
Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured.

Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines.

Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.

키워드

Yukgunja-tang tablet (YGJT-T); lipopolysaccharide (LPS); pro-inflammatory cytokines; acute gastritis; anti-inflammatory; anti-gastritis

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