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미숙감귤의 항산화 및 항노화 활성에 대한 평가

Evaluation on antioxidative and antiaging effect of immature fruits from Citrus unshiu Markovich

대한본초학회지 2021년 36권 1호 p.77 ~ 85
최문열, 박해진, 김재수, 김미려,
소속 상세정보
최문열 ( Choi Moon-Yeol ) - Daegu Haany University College of Korean Medicine Department of Herbal Pharmacology
박해진 ( Park Hae-Jin ) - DHU Bio Convergence Testing Center
김재수 ( Kim Jae-Soo ) - Daegu Haany University College of Korean Medicine Department of Acupuncture & Moxibustion Medicine
김미려 ( Kim Mi-Ryeo ) - Daegu Haany University College of Korean Medicine Department of Herbal Pharmacology

Abstract


Objective : This study was designed to investigate effect of immature fruits from Citrus unshiu Markovich to mature them on antioxidative and antiaging-related enzyme activities in vitro.

Methods : Fruits from Open field-cultivated C. unshiu Markovich (ripe and unripe fruits of Citri) made a purchase in Jeju island. We measured total polyphenol and flavonoid contents in mature C. unshiu Markovich (MC) and immature C. unshiu Markovich (IMC) 70% ethanol extract. DPPH free radical, ABTS radical, and hydroxyl radical scavenging activities in both extracts were determined. Also, reducing power, SOD-like activities, elastase inhibition, and collagenase inhibition activities of these extract were assayed at 5, 1, 0.5, and 0.1 ㎎/㎖.

Results : The total polyphenol contents in the ethanol extract of IMC were higher than those of MC (26.99 ± 0.89 ㎎/TAEg). And total flavonoid contents in them were very similar to both extract (MC; 7.25 ± 0.4 ㎎/RUEg and IMC; 7.44 ± 0.18 ㎎/RUEg). DPPH free radical scavenging activities, ABTS radical scavenging capacity, reducing power, and SOD-like activities of IMC-treated group showed significant increase compared to those of MC group in all treated concentrations. Particularly, DPPH free radical scavenging and elastase inhibition activities in IMC-treated group at 5 ㎎/㎖ showed similar with positive control group. Besides, IMC extract at 5 ㎎/㎖ concentration surpassed positive control (BHA or Vit. C) in SOD-like activities and ABTS radical scavenging capacity.

Conclusion : These results suggest that IMC ethanol extract may be used as a natural antioxidant and a antiaging material for development of functional foods and cosmeceuticals.

키워드

Anti-oxidant; anti-aging; Citrus unshiu Markovich; immature; total polyphenol contents

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