잠시만 기다려 주세요. 로딩중입니다.

맥문동 열수 추출물이 Poly I:C를 처리한 폐암세포주의 사멸 및 염증성 사이토카인 발현에 미치는 영향

Effects of Liriope muscari Water Extracts on the Cell Death and Inflammatory Cytokine Expression of Poly I:C-treated Lung Carcinoma Cells

대한본초학회지 2021년 36권 1호 p.97 ~ 102
강다연, 조남준, 렌친핸드, 이보희, 김은미, 남명수, 김기광,
소속 상세정보
강다연 ( Kang Da-Yeon ) - Chungnam National University Department of Biochemistry
조남준 ( Cho Nam-Joon ) - Chungnam National University Department of Biochemistry
렌친핸드 ( Renchinkhand Gereltuya ) - Chungnam National University Division of Animal Resoures Science
이보희 ( Lee Bo-Hee ) - Chungcheongnam-do Agricultural Research and Extension Services Gogi Berry Research Institute
김은미 ( Kim Eun-Mi ) - Korea Institute of Toxicology Department of Predictive Toxicology
남명수 ( Nam Myoung-Soo ) - Chungnam National University Division of Animal Resoures Science
김기광 ( Kim Kee-Kwang ) - Chungnam National University Department of Biochemistry

Abstract


Objectives : Virus infection through the respiratory tract causes various inflammatory diseases such as pneumonia, cystic fibrosis, and obstructive pulmonary disease, causing enormous social damage. Therefore, it is very important to develop a treatment and prevention of infectious diseases. In this study, we investigated the effect of water extracts of Liriope muscari (WELM), known to improve lung function, on the inflammatory response of lung carcinoma cell line A549 cells induced by the viral double stranded RNA mimetic Polyinosinic:polycytidylic acid (Poly I:C).

Methods : The cell viability by WELM treatment was analyzed using MTS assay in A549 cells. After inducing an inflammatory response to WELM-treated A549 cells with Poly I:C, the degree of apoptosis was confirmed through bright field microscopy. Interferon beta (IFN-β) mRNA expression level in A549 cells was analyzed by quantitative reverse transcription PCR (qRT-PCR).

Results : WELM treatment has no significant effect on cell viability of A549 cells. We confirmed that pre-treatment of WELM effectively reduces the Poly I:C-induced apoptotic cell death in A549 cells. In addition, it was confirmed that the mRNA expression level of IFN-β, a pro-inflammatory cytokine increased by Poly I:C treatment, was significantly suppressed by WELM treatment in A549 cells.

Conclusions : These results provide the evidence that WELM is effective at inhibiting inflammation on respiratory viral infections and suggest that Liriope muscari might be a valuable natural substance in the prevention and treatment of infectious diseases

키워드

Liriope muscari; Poly I:C; A549 cells; Apoptosis; Inflammation; IFN-β

원문 및 링크아웃 정보

 

등재저널 정보