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면화자 정유의 기억력 손상 완화 효과

Gossypii Semen oil alleviates memory dysfunction in scopolamine-treated mice

대한본초학회지 2021년 36권 2호 p.1 ~ 9
이지혜, 정은미, 이은홍, 장귀영, 서경혜, 김미려, 정지욱,
소속 상세정보
이지혜 ( Lee Ji-Hye ) - Daegu Haany University College of Herbal Bio-Industry Division of Bio-technology and Convergence
정은미 ( Jung Eun-Mi ) - Daegu Haany University College of Herbal Bio-Industry Division of Bio-technology and Convergence
이은홍 ( Lee Eun-Hong ) - Daegu Haany University College of Herbal Bio-Industry Division of Bio-technology and Convergence
장귀영 ( Jang Gwi-Yeong ) - RDA NIHHS Department of Herbal Crop Research
서경혜 ( Seo Kyung-Hye ) - RDA NIHHS Department of Herbal Crop Research
김미려 ( Kim Mi-Ryeo ) - Daegu Haany University College of Korean Medicine Department of Herbal Pharmacology
정지욱 ( Jung Ji-Wook ) - Daegu Haany University College of Herbal Bio-Industry Division of Bio-technology and Convergence

Abstract


Objectives : Gossypium arboreum (cotton) is traditionally used to treat various health disorders. However, anti-amnesic effect of G. arboreum has not been reported. The objective of this study was to investigate in-vivo the anti-amnesic effects along with in vitro antioxidant and acetylcholinesterase (AChE) inhibition potential in G.
arboreum seed essential oil.

Methods : The essential oil of G. arboreum obtained by solid phase microextraction (SPME) techniques were identified by gas chromatography-mass spectroscopy (GC-MS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were performed to determine the antioxidant activity at various concentrations (312.5, 625, 1250, 2500, 5000, 10000 ㎍/㎖. Y-maze, passive avoidance and Morris water maze tests were carried out to evaluate improved effect on scopolamine (1 ㎎/㎏)-induced memory dysfunction at the dose level of 50, 100 and 200 ㎎/㎏. Donepezil (5 ㎎/㎏) was used as a positive drug control. We performed acetylcholinesterase (AChE) activity assay in ex vivo.

Results : Five volatile compounds were identified in G. arboreum. The assays of DPPH and ABTS revealed that G.
arboreum increased antioxidant activity in a dose-dependent manner. G. arboreum ameliorated the percent of spontaneous alternation in the Y-maze test, shortened step-through latency in the passive avoidance test, and increased swimming time in the target zone in the Morris water maze test. In addition, G. arboreum inhibited the AChE activity.

Conclusions : Based on these findings, G. arboreum may aid in the prevention and treatment of learning and memory-deficit disorders through antioxidant and AChE inhibitory activities.

키워드

Scopolamine; Memory; Essential oil; Cotton seed; Gossypii Semen

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