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Burkholderia thailandensis outer membrane vesicles exert antimicrobial activity against drug-resistant and competitor microbial species

Journal of Microbiology 2020년 58권 7호 p.550 ~ 562
 ( Wang Yihui ) - Tulane University School of Medicine Department of Microbiology and Immunology

 ( Hoffmann Joseph P. ) - Tulane University School of Medicine Department of Microbiology and Immunology
 ( Chou Chau-Wen ) - University of Georgia Franklin College of Arts and Sciences Department of Chemistry
 ( zu Bentrup Kerstin Honer ) - Tulane University School of Medicine Department of Microbiology and Immunology
 ( Fuselier Joseph A. ) - Tulane University School of Medicine Department of Medicine
 ( Bitoun Jacob P. ) - Tulane University School of Medicine Department of Microbiology and Immunology
 ( Wimley William C. ) - Tulane University School of Medicine Department of Biochemistry and Molecular Biology
 ( Morici Lisa A. ) - Tulane University School of Medicine Department of Microbiology and Immunology

Abstract


Gram-negative bacteria secrete outer membrane vesicles (OMVs) that play critical roles in intraspecies, interspecies, and bacteria-environment interactions. Some OMVs, such as those produced by Pseudomonas aeruginosa, have previously been shown to possess antimicrobial activity against competitor species. In the current study, we demonstrate that OMVs from Burkholderia thailandensis inhibit the growth of drug-sensitive and drug-resistant bacteria and fungi. We show that a number of antimicrobial compounds, including peptidoglycan hydrolases, 4-hydroxy-3-methyl-2-(2-non-enyl)-quinoline (HMNQ) and long-chain rhamnolipid are present in or tightly associate with B. thailandensis OMVs. Furthermore, we demonstrate that HMNQ and rhamnolipid possess antimicrobial and antibiofilm properties against methicillin-resistant Staphylococcus aureus (MRSA). These findings indicate that B. thailandensis secretes antimicrobial OMVs that may impart a survival advantage by eliminating competition. In addition, bacterial OMVs may represent an untapped resource of novel therapeutics effective against bio-film-forming and multidrug-resistant organisms.

키워드

antimicrobial resistance; outer membrane vesicles; biofilm; bacteria; fungi
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