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Endophytic bacterial and fungal microbiota in different cultivars of cassava (Manihot esculenta Crantz)

Journal of Microbiology 2020년 58권 7호 p.614 ~ 623
 ( Li Hong ) - Hainan University School of Life and Pharmaceutical

 ( Yan Chengliang ) - Hainan University School of Tropical Crops
 ( Tang Yanqiong ) - Hainan University School of Life and Pharmaceutical
 ( Ma Xiang ) - Hainan University School of Life and Pharmaceutical
 ( Chen Yinhua ) - Hainan University School of Tropical Crops
 ( Chen Songbi ) - Chinese Academy of Tropical Agricultural Sciences Tropical Crops Genetic Resources Institute
 ( Lin Min ) - Chinese Academy of Agricultural Sciences Biotechnology Research Institute
 ( Liu Zhu ) - Hainan University School of Life and Pharmaceutical

Abstract


Endophytes colonize tissues of healthy host plants and play a crucial role in plant growth and development. However, little attention has been paid to the endophytes of tuber crops such as cassava, which is used as a staple food by approximately 800 million people worldwide. This study aimed to elucidate the diversity and composition of endophytic bacterial and fungal communities in different cassava cultivars using high-throughput sequencing. Although no significant differences in richness or diversity were observed among the different cassava cultivars, the community compositions were diverse. Two cultivars (SC124 and SC205) tolerant to root rot exhibited similar community compositions, while two other cultivars (SC10 and SC5), which are moderately and highly susceptible to root rot, respectively, harboured similar community compositions. Proteobacteria, Firmicutes, and Ascomycota dominated the endophyte assemblages, with Weissella, Serratia, Lasiodiplodia, Fusarium, and Diaporthe being the predominant genera. The differentially abundant taxonomic clades between the tolerant and susceptible cultivars were mainly rare taxa, such as Lachnoclostridium_5, Rhizobium, Lampropedia, and Stenotrophomonas. These seemed to be key genera that affected the susceptibility of cassava to root rot. Moreover, the comparison of KEGG functional profiles revealed that ‘Environmental adaptation’ category was significantly enriched in the tolerant cultivars, while ‘Infectious diseases: Parasitic’ category was significantly enriched in the susceptible cultivars. The present findings open opportunities for further studies on the roles of endophytes in the susceptibility of plants to diseases.

키워드

endophytic bacteria; endophytic fungi; cassava; susceptibility; high-throughput sequencing
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