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A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

Journal of Microbiology 2020년 58권 10호 p.823 ~ 831
정광우, 이경태, 반용선,
소속 상세정보
정광우 ( Jung Kwang-Woo ) - Korea Atomic Energy Research Institute Radiation Research Division
이경태 ( Lee Kyung-Tae ) - Yonsei University College of Life Science and Biotechnology Department of Biotechnology
반용선 ( Bahn Yong-Sun ) - Yonsei University College of Life Science and Biotechnology Department of Biotechnology

Abstract


Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

키워드

fungal pathogens; quantitative PCR; virulence; mutant library

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