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Roles of Dhh1 RNA helicase in yeast filamentous growth: Analysis of N-terminal phosphorylation residues and ATPase domains

Journal of Microbiology 2020년 58권 10호 p.853 ~ 858
이은지, 정대희, 김진미,
소속 상세정보
이은지 ( Lee Eun-Ji ) - Chungnam National University College of Bioscience and Biotechnology Department of Microbiology and Molecular Biology
정대희 ( Jung Dae-Hee ) - Chungnam National University College of Bioscience and Biotechnology Department of Microbiology and Molecular Biology
김진미 ( Kim Jin-Mi ) - Chungnam National University College of Bioscience and Biotechnology Department of Microbiology and Molecular Biology

Abstract


In yeast Saccharomyces cerevisiae, the Dhh1 protein, a member of the DEAD-box RNA helicase, stimulates Dcp2/Dcp1-mediated mRNA decapping and functions as a general translation repressor. Dhh1 also positively regulates translation of a selected set of mRNAs, including Ste12, a transcription factor for yeast mating and pseudohyphal growth. Given the diverse functions of Dhh1, we investigated whether the putative phosphorylation sites or the conserved motifs for the DEAD-box RNA helicases were crucial in the regulatory roles of Dhh1 during pseudohyphal growth. Mutations in the ATPase A or B motif (DHH1-K96R or DHH1-D195A) showed significant defects in pseudohyphal colony morphology and agar invasive phenotypes. The N-terminal phospho-mimetic mutation, DHH1-T16E, showed defects in pseudohyphal phenotypes. Decreased levels of Ste12 protein were also observed in these pseudohyphal-defective mutant cells under filamentous-inducing low nitrogen conditions. We suggest that the ATPase motifs and the Thr16 phosphorylation site of Dhh1 are crucial to its regulatory roles in pseudohyphal growth under low nitrogen conditions.

키워드

pseudohyphal growth; Dhh1; Ste12 expression; ATPase domain; phosphorylation

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