잠시만 기다려 주세요. 로딩중입니다.

Quantitative Analysis of Microperfusion in Contrast-Induced Nephropathy Using Contrast-Enhanced Ultrasound: An Animal Study

Korean Journal of Radiology 2021년 22권 5호 p.801 ~ 810
서니은, 오혜원, 오형중, 정용은,
소속 상세정보
서니은 ( Seo Ni-Eun ) - Yonsei University College of Medicine Severance Hospital Department of Radiology
오혜원 ( Oh Hye-Won ) - Yonsei University College of Medicine Severance Hospital Department of Radiology
오형중 ( Oh Hyung-Jung ) - Sheikh Khalifa Specialty Hospital Department of Nephrology
정용은 ( Chung Yong-Eun ) - Yonsei University College of Medicine Severance Hospital Department of Radiology

Abstract


Objective: To investigate imaging biomarkers of microperfusion in contrast-induced nephropathy (CIN) using contrast-enhanced ultrasound (CEUS).

Materials and Methods: The CIN model was fabricated by administering indomethacin (10 mg/kg), L-NAME (15 mg/kg), and iopamidol (10 mL/kg) to Sprague-Dawley rats. After 24 hours, CEUS was performed on CIN (n = 6) and control (n = 6) rats with sulphur hexafluoride microbubbles (SonoVue). From time-intensity curves obtained from the kidney arriving time (AT), acceleration time (AC), time to peak (TTP), and peak enhancement (PE) were measured and compared between the groups. After CEUS, the rats were sacrificed, and cell apoptosis markers were evaluated to confirm the development of CIN.

Results: Among CEUS parameters, AT (7.8 ± 1.6 vs. 4.2 ± 0.5 s, p = 0.002), AC (4.7 ± 1.4 vs. 2.0 ± 0.4 s, p = 0.002), and TTP (12.5 ± 2.9 vs. 6.2 ± 0.6 s, p = 0.002) were significantly prolonged in the CIN group compared to controls. PE was significantly higher in the control group than in the CIN group (17.1 ± 1.9 vs. 12.2 ± 2.0 dB, p = 0.004). In kidney tissue, mRNA and protein levels of the apoptotic makers were significantly higher in the CIN group than in the control group (p = 0.003 and p = 0.002).

Conclusion: CEUS parameters can be used as imaging biomarkers for microperfusion in CIN. In rats with CIN, AT, AC, and TTP were significantly prolonged, while PE was significantly lower compared to controls.

키워드

Contrast media; Acute kidney injury; Perfusion; Contrast-enhanced ultrasonography; Animal experimentation

원문 및 링크아웃 정보

  

등재저널 정보