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Systematic Review on Application of Whey Towards Production of Galacto-oligosaccharide Using β-Galactosidase Enzyme from Pichia pastoris

한국식품위생안전성학회지 2020년 35권 4호 p.304 ~ 311
Ramachandran C., 오덕환,
소속 상세정보
 ( Ramachandran C. ) - Kangwon National University Department of Food Science and Biotechnology
오덕환 ( Oh Deog-Hwan ) - Kangwon National University Department of Food Science and Biotechnology

Abstract


Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut, in addition to having various applications in the food industry. GOS are generally produced from lactose in a reaction catalyzed by ß-galactosidase. Synthesis of GOS from whey permeate (WP) (ultrafiltration of whey, concentrated then spray dried) using surface engineered β-galactosidase in Pichia pastoris (P. pastoris) is a novel method to convert waste into a valuable product. Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them to functional components of cells. Surface engineered cells have many potential uses. The Flo1p flocculation functional domain, thought to be located near the N terminus, recognizes and adheres non-covalently to cell-wall components such as α-mannan carbohydrates, causing reversible aggregation of cells into flocs.

키워드

Galactooligosaccharides; ß-Galactosidase; Whey; Mannan; Flocculation

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