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Prevalence and antimicrobial resistance of Klebsiella species isolated from clinically ill companion animals

Journal of Veterinary Science 2021년 22권 2호 p.17 ~ 17
이단, 오재영, Sum Samuth, 박희명,
소속 상세정보
이단 ( Lee Dan ) - Konkuk University College of Veterinary Medicine Department of Veterinary Internal Medicine
오재영 ( Oh Jae-Young ) - Konkuk University College of Veterinary Medicine Department of Veterinary Internal Medicine
 ( Sum Samuth ) - Konkuk University College of Veterinary Medicine Department of Veterinary Internal Medicine
박희명 ( Park Hee-Myung ) - Konkuk University College of Veterinary Medicine Department of Veterinary Internal Medicine

Abstract


Background: Klebsiella spp. is an important conditional pathogen in humans and animals. However, due to the indiscriminate use of antibiotics, the incidence of antimicrobial resistance has increased.

Objectives: The purpose of this study was to investigate antimicrobial resistance in strains of Klebsiella strains and the phylogenetic relatedness of extended-spectrum cephalosporin (ESC)-resistance among Klebsiella strains isolated from clinically ill companion animals.

Methods: A total of 336 clinical specimens were collected from animal hospitals. Identification of Klebsiella species, determination of minimum inhibitory concentrations, detection of ESC resistance genes, polymerase chain reaction-based replicon typing of plasmids by conjugation, and multilocus sequence typing were performed.

Results: Forty-three Klebsiella strains were isolated and, subsequently, 28 were identified as K. pneumoniae, 11 as K. oxytoca, and 4 as K. aerogenes. Eleven strains were isolated from feces, followed by 10 from ear, 7 from the nasal cavity, 6 from urine, 5 from genitals, and 4 from skin. Klebsiella isolates showed more than 40% resistance to penicillin, cephalosporin, fluoroquinolone, and aminoglycoside. ESCresistance genes, CTX-M groups (CTX-M-3, CTX-M-15, and CTX-M-65), and AmpC (CMY-2 and DHA-1) were most common in the K. pneumoniae strains. Some K. pneumoniae carrying CTX-M or AmpC were transferred via IncFII plasmids. Two sequence types, ST709 and ST307, from K. pneumoniae were most common.

Conclusions: In conclusion, this is the first report on the prevalence, ESCresistance genotypes, and sequence types of Klebsiella strains isolated from clinically ill companion animals. The combination of infectious diseases and antimicrobial resistance by Klebsiella in companion animals suggest that, in clinical veterinary, antibiotic selection should be made carefully and in conjunction with the disease diagnosis.

키워드

Companion animals; Klebsiella species; extended-spectrum cephalosporins; multilocus sequence type (MLST)

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