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Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection

Journal of Veterinary Science 2021년 22권 2호 p.18 ~ 18
Reyes Alisha Wehdnesday Bernardo, Huy Tran Xuan Ngoc, Vu Son Hai, 김현진, 이진주, 최정수, 이전화, 김석,
소속 상세정보
 ( Reyes Alisha Wehdnesday Bernardo ) - Gyeongsang National University College of Veterinary Medicine Institute of Animal Medicine
 ( Huy Tran Xuan Ngoc ) - Gyeongsang National University College of Veterinary Medicine Institute of Animal Medicine
 ( Vu Son Hai ) - Gyeongsang National University College of Veterinary Medicine Institute of Animal Medicine
김현진 ( Kim Hyun-Jin ) - Gyeongsang National University College of Veterinary Medicine Institute of Animal Medicine
이진주 ( Lee Jin-Ju ) - Animal and Plant Quarantine Agency
최정수 ( Choi Jeong-Soo ) - Animal and Plant Quarantine Agency
이전화 ( Lee John-Hwa ) - Chonbuk National University College of Veterinary Medicine
김석 ( Kim Suk ) - Gyeongsang National University College of Veterinary Medicine Institute of Animal Medicine

Abstract


Background: We previously elucidated the protective mechanism of Korean red ginseng oil (RGO) against Brucella abortus infection, and our phytochemical analysis revealed that palmitic acid (PA) was an abundant component of RGO. Consequently, we investigated the contribution of PA against B. abortus.

Objectives: We aimed to investigate the efficacy of PA against B. abortus infection using a murine cell line and a murine model.

Methods: Cell viability, bactericidal, internalization, and intracellular replication, western blot, nitric oxide (NO), and superoxide (O2-) analyses and flow cytometry were performed to determine the effects of PA on the progression of B. abortus infection in macrophages. Flow cytometry for cytokine analysis of serum samples and bacterial counts from the spleens were performed to determine the effect of PA in a mouse model.

Results: PA did not affect the growth of B. abortus. PA treatment in macrophages did not change B. abortus uptake but it did attenuate the intracellular survivability of B. abortus. Incubation of cells with PA resulted in a modest increase in sirtuin 1 (SIRT1) expression. Compared to control cells, reduced nitrite accumulation, augmented O2-, and enhanced pro-inflammatory cytokine production were observed in PA-treated B. abortus-infected cells. Mice orally treated with PA displayed a decreased serum interleukin-10 level and enhanced bacterial resistance.

Conclusions: Our results suggest that PA participates in the control of B. abortus within murine macrophages, and the in vivo study results confirm its efficacy against the infection. However, further investigations are encouraged to completely characterize the mechanisms involved in the inhibition of B. abortus infection by fatty acids.

키워드

B. abortus; macrophages; palmitic acid; spleen

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