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Single-nucleotide polymorphism-based epidemiological analysis of Korean Mycobacterium bovis isolates

Journal of Veterinary Science 2021년 22권 2호 p.24 ~ 24
김태운, 장윤호, 정민규, 서윤정, 박찬호, 강신석, 이영주, 최정수, 윤순식, 김재명,
소속 상세정보
김태운 ( Kim Tae-Woon ) - Animal and Plant Quarantine Agency
장윤호 ( Jang Yun-Ho ) - Animal and Plant Quarantine Agency
정민규 ( Jeong Min-Kyu ) - Animal and Plant Quarantine Agency
서윤정 ( Seo Yoon-Jeong ) - Animal and Plant Quarantine Agency
박찬호 ( Park Chan-Ho ) - Gangwondo Livestock and Veterinary Service
강신석 ( Kang Sin-Seok ) - Chungcheongbukdo Livestock and Veterinary Service
이영주 ( Lee Young-Ju ) - Kyungpook National University College of Veterinary Medicine
최정수 ( Choi Jeong-Soo ) - Animal and Plant Quarantine Agency
윤순식 ( Yoon Soon-Seek ) - Animal and Plant Quarantine Agency
김재명 ( Kim Jae-Myung ) - Animal and Plant Quarantine Agency

Abstract


Background: Bovine tuberculosis (TB) is caused by Mycobacterium bovis, a well-known cause of zoonotic tuberculosis in cattle and deer, and has been investigated in many physiological and molecular studies. However, detailed genome-level studies of M. bovis have not been performed in Korea.

Objectives: To survey whole genome-wide single-nucleotide polymorphism (SNP) variants in Korean M. bovis field isolates and to define M. bovis groups in Korea by comparing SNP typing with spoligotyping and variable number tandem repeat typing.

Methods: A total of 46 M. bovis field isolates, isolated from laryngopharyngeal lymph nodes and lungs of Korean cattle, wild boar, and Korean water deer, were used to identify SNPs by performing whole-genome sequencing. SNP sites were confirmed via polymerase chain reaction using 87 primer pairs.

Results: We identified 34 SNP sites with different frequencies across M. bovis isolates, and performed SNP typing and epidemiological analysis, which divided the 46 field isolates into 16 subtypes.

Conclusions: Through SNP analysis, detailed differences in samples with identical spoligotypes could be detected. SNP analysis is, therefore, a useful epidemiological tracing tool that could enable better management of bovine TB, thus preventing further outbreaks and reducing the impact of this disease.

키워드

Bovine tuberculosis; Mycobacterium bovis; Single nucleotide polymorphism; spoligotype; variable number of tandem repeat

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