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Sputum Processing Method for Lateral Flow Immunochromatographic Assays to Detect Coronaviruses

Immune Network 2021년 21권 1호 p.11 ~ 11
강아람, 염민주, 김혜권, Yoon Sun-Woo, 정대균, 문형준, Lyoo Kwang-Soo, Na Woon-Sung, 송대섭,
소속 상세정보
강아람 ( Kang A-Ram ) - Korea University College of Pharmacy
염민주 ( Yeom Min-Joo ) - Korea University College of Pharmacy
김혜권 ( Kim Hye-Kwon ) - Chungbuk National University Department of Microbiology
 ( Yoon Sun-Woo ) - Korea Research Institute of Bioscience and Biotechnology
정대균 ( Jeong Dae-Gwin ) - Korea Research Institute of Bioscience and Biotechnology
문형준 ( Moon Hyong-Joon ) - Semyung University College of Healthcare and Biotechnology
 ( Lyoo Kwang-Soo ) - Chonbuk National University Korea Zoonosis Research Institute
 ( Na Woon-Sung ) - Chonnam National University College of Veterinary Medicine
송대섭 ( Song Dae-Sub ) - Korea University College of Pharmacy

Abstract


Coronavirus causes an infectious disease in various species and crosses the species barriers leading to the outbreak of zoonotic diseases. Due to the respiratory diseases are mainly caused in humans and viruses are replicated and excreted through the respiratory tract, the nasal fluid and sputum are mainly used for diagnosis. Early diagnosis of coronavirus plays an important role in preventing its spread and is essential for quarantine policies. For rapid decision and prompt triage of infected host, the immunochromatographic assay (ICA) has been widely used for point of care testing. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, it is necessary to use a mucolytic agent without affecting the antigens. In this study, we combined known mucolytic agents to lower the viscosity of sputum and applied that to alpha and beta coronavirus, porcine epidemic diarrhea virus (PEDV) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, spiked in sputum to find optimal pretreatment conditions. The pretreatment method using tris(2-carboxyethyl)phosphine (TCEP) and BSA was suitable for ICA diagnosis of sputum samples spiked with PEDV and MERS-CoV. This sensitive assay for the detection of coronavirus in sputum provides an useful information for the diagnosis of pathogen in low respiratory tract.

키워드

Coronavirus; Diagnosis; Sputum; Chromatography, Sensitivity

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