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Analysis of Mutant Isocitrate Dehydrogenase 1 Immunoexpression, Ki-67 and Programmed Death Ligand 1 in Diffuse Astrocytic Tumours : Study of Single Center in Bandung, Indonesia

Journal of Korean Neurosurgical Society 2021년 64권 1호 p.100 ~ 109
Bolly Hendrikus Masang Ban, Faried Ahmad, Hermanto Yulius, Lubis Billy Parulian, Tjahjono Firman Priguna, Hernowo Bethy Suryawathy, Arifin Muhammad Zafrullah,
소속 상세정보
 ( Bolly Hendrikus Masang Ban ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Neurosurgery
 ( Faried Ahmad ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Neurosurgery
 ( Hermanto Yulius ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Neurosurgery
 ( Lubis Billy Parulian ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Anatomical Pathology
 ( Tjahjono Firman Priguna ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Neurosurgery
 ( Hernowo Bethy Suryawathy ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Anatomical Pathology
 ( Arifin Muhammad Zafrullah ) - Universitas Padjadjaran-Dr. Hasan Sadikin Hospital Faculty of Medicine Department of Neurosurgery

Abstract


Objective: Diffuse astrocytic tumour (DAT) is a diffuse infiltrative astrocytoma tumour accompanied by molecular parameters such as the presence or absence of isocitrate dehydrogenase (IDH) gene mutations. Ki-67 is a marker for DAT proliferation, while programmed death ligand 1 (PD-L1) indicates an immune evasion mechanism. This study aimed to analyze the correlation among mutant IDH1 R132H, Ki-67, and PD-L1 immunoexpression in the DAT.

Methods: A cross-sectional study was carried out on 30 paraffin blocks of DAT cases. Paraffin block samples consist of grade II (n=14), grade III (n=8), and grade IV (n=8). In this study, the immunohistochemistry-staining of mutant IDH1 R132H, Ki-67, and PD-L1 were carried out to determine the frequency of DAT with IDH1 mutations.

Results: Our study shown the frequency of IDH1 mutations in grade II 50.0% (7/14), grade III 37.5% (3/8), and grade IV 12.5% (1/8). Our study also showed a difference in Ki-67 and PD-L1 expression between each the degree of DAT histopathology (p=0.0001 and p=0.002, respectively). There was an association between both mutant IDH1 R132H, and Ki-67 with PD-L1 expression in DAT (p=0.0087 and p=0.0049, respectively).

Conclusion: DAT with the mutant IDH1 is frequently observed in grade II and small number of grade III. The expression of wild type IDH1, Ki-67, and PD-L1 were found to be higher in high grade DAT (grade III and grade IV). There is a correlation between each of mutant IDH1 status and Ki-67 with PD-L1 expression in DAT.

키워드

Diffuse astrocytic tumor; Histopathology grade; Mutant IDH1 R132H; Ki-67; PD-L1

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