잠시만 기다려 주세요. 로딩중입니다.

Development and validation of multiplex PCR assay for differentiating tunas and billfishes

Food Science and Biotechnology 2021년 30권 4호 p.497 ~ 503
김나예슬, 박은지, 이서현, 문광호, 양지영, 김중범,
소속 상세정보
김나예슬 ( Kim Na-Ye-Seul ) - Suncheon National University Department of Food Science and Technology
박은지 ( Park Eun-Ji ) - Suncheon National University Department of Food Science and Technology
이서현 ( Lee Seo-Hyun ) - Suncheon National University Department of Food Science and Technology
문광호 ( Mun Kwang-Ho ) - Suncheon National University Department of Food Science and Technology
양지영 ( Yang Ji-Young ) - Pukyong National University Department of Food Science and Technology
김중범 ( Kim Jung-Beom ) - Suncheon National University Department of Food Science and Technology

Abstract


Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We developed and validated species-specific primers to prevent the adulteration of tunas by billfishes. Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control. Sensitivity was determined to be 5 ng for tunas and billfishes. A total of 50 samples were monitored: 49 for tunas and 1 for billfish. As a result of the monitoring, the fake tunas did not show due to the agreement between product name and the raw material of the wrapping paper. Our results indicate that the species-specific primers developed in this study are suitable for differentiating tunas and billfishes. The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.

키워드

Tunas; Billfishes; PCR assay; Validation; Monitoring

원문 및 링크아웃 정보

등재저널 정보