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Detection of GM Canola MS11, DP-073496-4, and MON88302 events using multiplex PCR coupled with capillary electrophoresis

Food Science and Biotechnology 2021년 30권 4호 p.565 ~ 570
이도근, 박지은, 김미주, 김현중, 김해영,
소속 상세정보
이도근 ( Lee Do-Geun ) - Kyung Hee University Department of Food Science and Biotechnology
박지은 ( Park Ji-Eun ) - Kyung Hee University Department of Food Science and Biotechnology
김미주 ( Kim Mi-Ju ) - Kyung Hee University Department of Food Science and Biotechnology
김현중 ( Kim Hyun-Joong ) - Mokpo National University Department of Food Engineering
김해영 ( Kim Hae-Yeong ) - Kyung Hee University Department of Food Science and Biotechnology

Abstract


As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events. To verify the specificity of event-specific primers, various GM crops of 3 GM soybean events, 6 GM maize events, 2 GM cotton events and 11 GM canola events were prepared. The limit of detection of the developed multiplex PCR was approximately 0.0125% for 3 GM canola events. Certified GM canola and stacked events were analyzed to validate the developed multiplex PCR. This study focuses on establishing multiplex PCR coupled with capillary electrophoresis for newly approved GM canola events and contributes to efficient monitoring GM canola samples in Korea.

키워드

Genetically modified canola; Event-specific detection; Multiplex polymerase chain reaction; Monitoring; Capillary electrophoresis

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