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UPLC-DAD-QTOF/MS를 이용한 국내산 생강(Zingiber officinale Rosc.) 잎과 줄기의 추출 용매별 플라보노이드 성분 분석

Comparative Analysis of the Flavonoid Content of Korean Ginger(Zingiber officinale Rosc.) Leaves and Stem by Solvent Extraction using UPLC-DAD-QTOF/MS

한국식품조리과학회지 2021년 37권 1호 p.81 ~ 92
남동건, 이수지, 임정연, 황경아, 최정숙, 임푸름,
소속 상세정보
남동건 ( Nam Dong-Geon ) - Rural Development Administration National Academy of Agricultural Sciences Functional Food & Nutrition Division
이수지 ( Lee Su-Ji ) - Rural Development Administration National Institute of Agricultural Sciences Food & Nutrition Division
임정연 ( Im Jeong-Yeon ) - Rural Development Administration National Institute of Agricultural Sciences Food & Nutrition Division
황경아 ( Hwang Kyung-A ) - Rural Development Administration National Institute of Agricultural Sciences Food & Nutrition Division
최정숙 ( Choe Jeong-Sook ) - Rural Development Administration National Academy of Agricultural Sciences Functional Food & Nutrition Division
임푸름 ( Im Pu-Reum ) - Rural Development Administration National Academy of Agricultural Sciences Functional Food & Nutrition Division

Abstract


Purpose: To investigate the main phenolic compound in Korean ginger (Zingiber officinale Rosc.) leaves and stem by isolating the flavonoids content through solvent extraction using UPLC-DAD-QTOF/MS.

Methods: The flavonol glycosides were extracted using ethanol and hot-water. Thirteen flavonol glycosides were identified by a comparison of the ultraviolet and mass spectra with data from a chemical library and published literature.

Results: Among these, novel compounds quercetin and kaempferol glycosides [M+H]+: quercetin 3-O-robinobioside-7-O-rhamnoside [m/z 757.2189], quercetin 3-O-rutinoside-7-O-rhamnoside [m/z 757.2184], quercetin 3-O-galactoside-7-O-rhamnoside [m/z 611.1623], quercetin 3-O-glucoside-7-O-rhamnoside [m/z 611.1617], quercetin 3-O-robinobioside[m/z 611.1621], quercetin 3-O-rutinoside [m/z 611.1608], quercetin 3-O-glucoside [m/z 465.1039], kaempferol 3-O-robinobioside-7-O-rhamnoside [m/z 741.2239], kaempferol 3-O-rutinoside-7-O-rhamnoside [m/z 741.2242], kaempferol 3-O-galactoside-7-O-rhamnoside [m/z 595.1667], kaempferol 3-O-robinobioside [m/z 595.1663], kaempferol 3-O-rutinoside [m/z 595.1660], kaempferol 3-O-glucoside [m/z 449.1088] were newly confirmed from the ginger leaves and stem. In particular, quercetin 3-O-glucoside (isoquercitrin) and quercetin 3-O-rutinoside (rutin) were confirmed as the major compounds in flavonols. Ethanol extraction of the ginger leaves and stem(213.47±2.60 and 59.88±0.63 mg/100 g dry weight) showed a higher flavonoid content than hot-water extracts (27.48±0.19 and 7.13±0.04 mg/100 g dry weight).
Conclusion: This study has shown that, the use of ethanol in the solvent extraction process can significantly increase the flavonoid content obtained from ginger leaves and stem. The flavonoids in Korean ginger could serve as a potential source of natural antioxidants for use in various industrial applications.

키워드

quercetin 3-O-rutinoside; Zingiber officinale Rosc.; leaf; stem; UPLC-DAD-QTOF/MS

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