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The Effects of Needle Electrode Electrical Stimulation on Cellular Necrosis Blocking the Forebrain after Induction of Ischemia

김성원, 이정숙, 박승규, 강한주, 김영수, 윤영대, 양회송, Lee Han-Gi, 김상수,
소속 상세정보
김성원 ( Kim Sung-Won ) - Hanyeong College
이정숙 ( Lee Jung-Sook ) - Dongju College
박승규 ( Park Seung-Gyu ) - Daebul University
강한주 ( Kang Han-Ju ) - Australia Action Physiotherapy and Rehab Hospital
김영수 ( Kim Yong-Soo ) - Shinsung University
윤영대 ( Yoon Young-Dae ) - Yonsei Semirae Hospital
양회송 ( Yang Hoe-Song ) - Suncheon Cheongnam College
 ( Lee Han-Gi ) - Masan University
김상수 ( Kim Sang-Soo ) - Daegu Health College

Abstract


This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related substances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-histochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.

키워드

NEES; ischemia; apoptosis; inflammation; necrosis; reperfusion

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