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The Effects of NEES on PARP Expression and Cell Death in Rat Cerebral Cortex After Ischemic Injury

김성원, 이정숙, 엄기매, 김지성, 이석희, 최유림, 김년준, 김보경, 조미숙, 박주현, 김순희,
소속 상세정보
김성원 ( Kim Sung-Won ) - Dongnam Health University
이정숙 ( Lee Jung-Sook ) - Dongju College
엄기매 ( Um Ki-Mai ) - Yeoju College
김지성 ( Kim Ji-Sung ) - Suwon Womens College
이석희 ( Lee Suk-Hee ) - Changwon College
최유림 ( Choi Yoo-Rim ) - Daegu Science College
김년준 ( Kim Nyeon-Jun ) - Pohang College
김보경 ( Kim Bo-Kyoung ) - International University of Korea
조미숙 ( Cho Mi-Suk ) - Nazarene University
박주현 ( Park Joo-Hyun ) - Sarang Hospital
김순희 ( Kim Soon-Hee ) - Yongin University

Abstract


The majority of strokes are caused by ischemia and result in brain tissue damage, leading to problems of the central nervous system including hemiparesis, dysfunction of language and consciousness, and dysfunction of perception. The purpose of this study was to investigate the effects of Poly(ADP-ribose) polymerase(PARP) on necrosis in neuronal cells that have undergone needle electrode electrical stimulation(NEES) prior to induction of ischemia. Ischemia was induced in male SD rats(body weight 300g) by occlusion of the common carotid artery for 5 min, after which the blood was reperfused. After induction of brain ischemia, NEES was applied to Zusanli(ST 36), at 12, 24 and 48 hours. Protein expression was investigated using immuno-reactive cells, which react to PARP antibodies in cerebral nerve cells, and Western blotting. The results were as follows: In the cerebral cortex, the number of PARP reactive cells after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. PARP expression after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. As a result, NEES showed the greatest effect on necrosis- related PARP immuno-reactive cells 24 hours after ischemia, indicating necrosis inhibition, blocking of neural cell death, and protection of neural cells. Based on the results of this study, NEES can be an effective method of treating dysfunction and improving function of neuronal cells in brain damage caused by ischemia.

키워드

Ischemia; Necrosis; Needle Electrode Electrical Stimulation; Stroke; Zusanli

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