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원유 세균수 검사장비의 정도관리를 위한 표준시료의 개발 및 평가

Development and evaluation of standard samples for quality control of automated total bacterial counter in raw milk

한국가축위생학회지 2020년 43권 3호 p.147 ~ 154
강혜정, 김진환, 변영섭, 이하나, 이혜영, 김지현, 홍세림, 김하영, 윤순식, 문진산,
소속 상세정보
강혜정 ( Kang Hye-Jeong ) - Animal and Plant Quarantine Agency Bacterial Disease Division
김진환 ( Kim Jin-Hwan ) - Animal and Plant Quarantine Agency Bacterial Disease Division
변영섭 ( Byun Yeong-Seob ) - Animal and Plant Quarantine Agency Bacterial Disease Division
이하나 ( Lee Ha-Na ) - Animal and Plant Quarantine Agency Bacterial Disease Division
이혜영 ( Lee Hye-Young ) - Animal and Plant Quarantine Agency Bacterial Disease Division
김지현 ( Kim Ji-Hyeon ) - Animal and Plant Quarantine Agency Bacterial Disease Division
홍세림 ( Hong Se-Rim ) - Animal and Plant Quarantine Agency Bacterial Disease Division
김하영 ( Kim Ha-Young ) - Animal and Plant Quarantine Agency Bacterial Disease Division
윤순식 ( Yoon Soon-Seek ) - Animal and Plant Quarantine Agency Bacterial Disease Division
문진산 ( Moon Jin-san ) - Animal and Plant Quarantine Agency Bacterial Disease Division

Abstract


Standard samples were prepared for this study, and applied for BactoScan TM and BactoCount TM in order for quality control evaluation for total bacterial count in raw milk. Accordingly, the preparation of two lots of standard samples for quality control were lyophilized, which contain Lactobacillus lactis. The standard samples were prepared into three different levels of bacterial counts, those were Low 30,000∼ 40,000, Medium 70,000∼90,000, High 150,000∼220,000 CFU/mL, respectively. Then, the proficiency tests were performed in total 19 laboratories for measuring total bacterial counts. The total bacterial counts in the standard samples showed 37∼42, 82∼105, 214∼240 CFU/mL in the first lot, and it showed 30∼36, 67∼75, 131∼163 CFU/mL in the second lot in low, medium and high levels, respectively. Based on these results, the absolute values of z-scores of six standard samples in 18 laboratories were ≤2, which means the samples are satisfactory. However, z-score in one laboratory was ≤3, which means the sample is questionable. Using two standard samples, the correlation between BactoScan TM and BactoCount TM was 0.9982, which means the results of total bacterial count measurement of both equipment were equivalent. Therefore, the standard samples manufactured in this study for quality control of total bacterial count using BactoScan TM and BactoCount TM in the raw milk could be applied to proficiency tests.

키워드

Proficiency test; Quality control; Raw milk; Standard sample; Total bacterial count

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