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Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis

한국가축위생학회지 2020년 43권 4호 p.201 ~ 209
홍이곤, Choi Woo-Jae, 노영혜, 안선민, 김은경, 최은희, 김단일,
소속 상세정보
홍이곤 ( Hong Lee-Gon ) - Seoul National University College of Veterinary Medicine
 ( Choi Woo-Jae ) - Seoul National University College of Veterinary Medicine
노영혜 ( Ro Young-Hye ) - Seoul National University College of Veterinary Medicine
안선민 ( Ahn Sun-Min ) - Seoul National University College of Veterinary Medicine
김은경 ( Kim Eun-Kyung ) - Seoul National University Farm Animal Clinical Training and Research Center
최은희 ( Choe Eun-Hee ) - Seoul National University Farm Animal Clinical Training and Research Center
김단일 ( Kim Dan-Il ) - Seoul National University College of Veterinary Medicine

Abstract


In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon- gamma (IFN-γ) is included in Korea’s diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM ⓡ ) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM ⓡ and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM ⓡ , respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM ⓡ kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TBFeron Plus kit was not inferior to BOVIGAM ⓡ in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.

키워드

Bovine tuberculosis; Gamma-interferon; Diagnosis; Delayed time; Lymphocyte

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