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In vivo quantification of mandibular bone remodeling and vascular changes in a Wistar rat model: A novel HR-MRI and micro-CT fusion technique

Imaging Science in Dentistry 2020년 50권 3호 p.199 ~ 208
Song Dandan, Shujaat Sohaib, Zhao Ruiting, Huang Yan, Shaheen Eman, Van Dessel Jeroen, Orhan Kaan, Velde Greetje Vande, Coropciuc Ruxandra, Pauwels Ruben, Politis Constantinus, Jacobs Reinhilde,
소속 상세정보
 ( Song Dandan ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Shujaat Sohaib ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Zhao Ruiting ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Huang Yan ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Shaheen Eman ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Van Dessel Jeroen ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Orhan Kaan ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Velde Greetje Vande ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Coropciuc Ruxandra ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Pauwels Ruben ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Politis Constantinus ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology
 ( Jacobs Reinhilde ) - KU Leuven Faculty of Medicine Department of Imaging and Pathology

Abstract


Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model.

Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared.

Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat.

Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

키워드

Vascular Remodeling; Radiology; Jaw; Rats

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