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Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

한국축산식품학회지 2020년 40권 5호 p.746 ~ 757
김경휘, 김재원, 김재곤, 채종표, 이진선, 윤성식,
소속 상세정보
김경휘 ( Kim Gyeong-Hwuii ) - Yonsei University Department of Biological Science and Technology and IPAID
김재원 ( Kim Jae-Won ) - CJ CheilJedang Institute of Biotechnology
김재곤 ( Kim Jae-Gon ) - Yonsei University Department of Biological Science and Technology and IPAID
채종표 ( Chae Jong-Pyo ) - CJ CheilJedang Institute of Biotechnology
이진선 ( Lee Jin-Sun ) - Yonsei University Department of Biological Science and Technology and IPAID
윤성식 ( Yoon Sung-Sik ) - Yonsei University Division of Biological Science and Technology

Abstract


Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae. One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4℃ and 55℃, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.

키워드

animal husbandry; animal source food; bacteriophage; enterotoxigenic Escherichia coli; genome annotation

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