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Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea

한국축산식품학회지 2020년 40권 5호 p.758 ~ 771
김용훈, 김한솔, 김석환, 김미경, 곽효선,
소속 상세정보
김용훈 ( Kim Yong-Hoon ) - Ministry of Food and Drug Safety National Institute of Food and Drug Safety Evaluation Food Microbiology Division
김한솔 ( Kim Han-Sol ) - Ministry of Food and Drug Safety National Institute of Food and Drug Safety Evaluation Food Microbiology Division
김석환 ( Kim Seok-Hwan ) - Ministry of Food and Drug Safety National Institute of Food and Drug Safety Evaluation Food Microbiology Division
김미경 ( Kim Mi-Gyeong ) - Ministry of Food and Drug Safety National Institute of Food and Drug Safety Evaluation Food Microbiology Division
곽효선 ( Kwak Hyo-Sun ) - Ministry of Food and Drug Safety National Institute of Food and Drug Safety Evaluation Food Microbiology Division

Abstract


This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus. Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA-positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.

키워드

antimicrobial resistance; methicillin-resistant Staphylococcus aureus (MRSA); multilocus sequence typing (MLST); spa typing; pulsed-field gel electrophoresis (PFGE)

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