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High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation

Clinical and Experimental Reproductive Medicine 2022년 49권 2호 p.149 ~ 158
Mohsenzadeh Mehdi, Khalili Mohammad Ali, Anbari Fatemeh, Vatanparast Mahboubeh,
소속 상세정보
 ( Mohsenzadeh Mehdi ) - Gerash University of Medical Sciences Gerash Al-Zahra Fertility Center
 ( Khalili Mohammad Ali ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute
 ( Anbari Fatemeh ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute
 ( Vatanparast Mahboubeh ) - Rafsanjan University of Medical Sciences Research Institute of Basic Medical Sciences Molecular Medicine Research Center

Abstract


Objective: Optimizing culture media for the incubation of immature oocytes is a vital strategy to increase the oocyte maturation rate during in vitro maturation (IVM) programs. This study evaluated the IVM and fertilization rates of human germinal vesicle (GV) and metaphase I (MI) oocytes using two different maturation media (commercial and homemade) with or without growth differentiation factor 9-β (GDF9-β). supplementation.

Methods: Immature oocytes from intracytoplasmic sperm injection (ICSI) cycles were collected and assigned to one of two IVM culture media (commercial or homemade; cleavage-stage base). After maturation, MII oocytes were examined under an inverted microscope for the presence of the polar body, zona pellucida (ZP) birefringence, and meiotic spindle (MS) visualization after maturation in four conditions (commercial or homemade medium, with or without GDF9-β. ICSI was done for matured oocytes, and fertilization was confirmed by the visualization of two distinct pronuclei and two polar bodies.

Results: No significant differences were found between the two culture media in terms of the time and rate of oocyte maturation or the rate of fertilization (p>0.05). Growth factor supplementation increased the 24-hour maturation rate for both GV and MI oocytes only in homemade medium. The maturation rate after 24 hours was higher for MI oocytes (p<0.05). Similar results were observed for MS visualization and ZP structure in both types of media (p>0.05).

Conclusion: Higher rates of oocyte maturation and fertilization were observed after application of homemade medium supplemented with GDF9-β. Therefore, this combination may be recommended as an alternative for clinical IVM programs.

키워드

Fertilization; Growth differentiation factor 9; In vitro oocyte maturation techniques; Spindle apparatus

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