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Hyaluronic acid의 분자량과 농도에 따른 차이가 Glucose Oxidase-mediated Peroxidase System 효소 활성에 미치는 영향

Influence of Hyaluronic Acids of Different Molecular Weights and Concentrations on the Enzymatic Activities of the Glucose Oxidase-mediated Peroxidase System

대한노년치의학회지 2020년 16권 1호 p.1 ~ 7
김지훈, 장지연, 김윤영, 고홍섭,
소속 상세정보
김지훈 ( Kim Ji-Hoon ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
장지연 ( Chang Ji-Youn ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
김윤영 ( Kim Yoon-Young ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
고홍섭 ( Kho Hong-Seop ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis

Abstract


Hyaluronic acid has been used as a constituent of oral health care products. The purpose of this study was to investigate
influences of hyaluronic acids of different molecular weights and concentrations on the enzymatic activities of the glucose
oxidase-mediated peroxidase system. Hyaluronic acids of four different molecular weights (10 kDa, 100 kDa, 1 MDa, and 2
MDa) and reagents containing the glucose oxidase-mediated peroxidase system were used for assays. The enzymatic activity of the glucose oxidase-mediated peroxidase system was examined by measuring oxidized o-dianisidine production. The four kinds of hyaluronic acids at 0.5 mg/mL did not affect the enzymatic activities of the glucose oxidase-mediated peroxidase system. Among the three kinds of specific molecular weight-concentation conditions (100 kDa-5.0 mg/mL, 1 MDa-0.5 mg/ mL, and 2 MDa-0.2 mg/mL) showing viscosity values similar to human whole saliva, only the 100 kDa-5.0 mg/mL condition inhibited the enzymatic activities of the glucose oxidase-mediated peroxidase system significantly (P < 0.05). Mixtures of hyaluronic acids with different molecular weights did not affect the enzymatic activities of the glucose oxidase-mediated peroxidase system. In conclusion, hyaluronic acids and the glucose oxidase-mediated peroxidase system could be used together in oral health care products. However, possible interactions between the molecules need to be considered.

키워드

Hyaluronic acid; Molecular weight; Glucose oxidase-mediated peroxidase

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