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N-type Cav channel inhibition by spider venom peptide of Argiope bruennichi

Molecular & Cellular Toxicology 2021년 17권 1호 p.59 ~ 67
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Abstract


Background: The spider venom is composed of various bioactive peptides and has well-known physiological characteristics, such as cytolytic and neurotoxic activities. However, there have been few studies on neurotoxic peptides derived from domestic indigenous spiders in Korea.

Objective: The study aimed to characterize and identify the venom peptide through genomic analysis from the domestic indigenous spider, Argiope bruennichi. Toxin-like peptides were selected using homology analysis against well-known toxin peptides along with the secondary structural characterization analysis by cysteine pattern and disulfide bonds. Modulation of voltage-gated calcium (Cav) channel was measured by Ca2+ influx using fluorescence dye.

Results: We found that a novel peptide Aranetoxin-Ab1a significantly reduced intracellular Ca2+ levels in human neuroblastoma cell line SH-SY5Y via the inactivation of N-type Cav channels. Decreased intracellular Ca2+ influx by the treatment of the peptide inactivated the extracellular-regulated protein kinase 1/2 and cAMP-response factor binding protein pathway.

Conclusion: Our results provide beneficial information for the potential development of drugs utilizing novel peptide derived from spider venom, showing the inhibition of N-type Cav by the peptide.

키워드

Spider venom; Argiope bruennichi; Transcriptomics; RNA-sequencing; In silico analysis; Neurotoxicity

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