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꽃송이버섯 추출물이 RAW 264.7 세포에서 TNF-α, iNOS, IL-1β 유전자 발현에 미치는 영향

The Effect of Water Extracted from Sparassis crispa on the Expression of TNF-α, iNOS and IL-1β Genes in RAW 264.7 Cells

대한통합의학회지 2021년 9권 1호 p.163 ~ 171
한효상,
소속 상세정보
한효상 ( Han Hyo-Sang ) - Joongbu University Department of Health Administration

Abstract

목적 : 급속한 산업화로 인해 다양한 환경 오염원이 발생하여 인체와 접촉하는 과정에서 자극과 염증을 일으켜 건강에 악영향을 미치게 된다. 따라서 우리는 꽃송이버섯의 물 추출물이 RAW 264.7 세포의 기능에 관여하는 반응 유전자의 발현에 미치는 영향을 조사하였다.

방법 : 꽃송이버섯 열수 추출물을 이용하여 RAW 264.7 세포가 세포 활성도, 항산화, mRNA 발현에 미치는 영향을 조사하였다.

결과 : 꽃송이버섯의 다양한 농도의 열수 추출물로 처리 후 RAW 264.7 세포에 대한 MTS 검사를 실시했다. 꽃송이버섯버섯의 열수 추출물을 이용 RAW 264.7세포를 6시간 동안 200μg / ml 농도로 처리하여 TNF-α, iNOS, IL-1β의 mRNA 발현이 촉진되었다.

결론 : 다양한 농도의 꽃송이버섯 열수 추출물을 RAW 264.7 세포에 적용한 후 꽃송이 버섯의 항산화 활성이 나타났다.

Purpose: The purpose of this study was to examine the anti-inflammatory effects of Sparassis crispa (SC). SC is a well-known traditional herbal remedy and its mushroom is used for treatment of inflammation. Many diseases that are increasing recently have characteristics of inflammatory diseases. Researchers are finding bioactive substances from natural products that can promote treatment and prevention of inflammation. We investigated the effect of water extracted from SC on the expression of effector genes involved in the function of RAW 264.7 cells.

Methods: Effects of RAW 264.7 cells on cell viability, antioxidation, and mRNA expression were examined using water extracts from SC. A 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to determine the effect of water extracts from SC on cell viability in RAW 264.7 cells. Inflammation of RAW 264.7 cells induced by lipopolysaccharide (LPS) treatment and expression levels of inflammatory cytokine TNF-α, iNOS and IL-1β gene were analyzed using quantitative reverse transcription PCR (qRT-PCR) analysis.

Results: The MTS assay was performed on RAW 264.7 cells after treatment with various concentrations of water extracts of SC. Treatment of RAW 264.7 cells with water extracts from LPS and SC at a concentration of 0.125, 0.5 ㎎/㎖ for twentyfour hours promoted mRNA expression of TNF-α, iNOS and IL-1β.

Conclusion: MTS assay was applied to RAW 264.7 cells after various concentrations of water extracts of SC. Through experimental demonstration of anti-oxidant and anti-inflammatory effects of water extracts from SC, we suggest that SC is a valuable material for the prevention and treatment of various inflammatory diseases.

키워드

꽃송이버섯; 대식세포; 세포독성; 지질다당체; 항염증
anti-inflammation; lipopolysaccharide; MTS assay; RAW 264.7 cell; Sparassis crispa

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