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Molecular and Histologic Evidence of Novel Erectile Dysfunction Rat Model as an Aging Atherosclerosis Model: A Preliminary Study

The World Journal of Men′s Health 2020년 38권 3호 p.345 ~ 352
김재헌, 심지성, 김종욱, 두승환, 배재현, 이주한, 송윤섭, 김제종, 문두건,
소속 상세정보
김재헌 ( Kim Jae-Heon ) - Soonchunhyang University College of Medicine Soonchunhyang University Hospital Department of Urology
심지성 ( Shim Ji-Sung ) - Korea University College of Medicine Department of Urology
김종욱 ( Kim Jong-Wook ) - Korea University College of Medicine Department of Urology
두승환 ( Doo Seung-Whan ) - Soonchunhyang University College of Medicine Soonchunhyang University Hospital Department of Urology
배재현 ( Bae Jae-Hyun ) - Korea University College of Medicine Department of Urology
이주한 ( Lee Ju-Han ) - Korea University College of Medicine Korea University Ansan Hospital Department of Pathology
송윤섭 ( Song Yun-Seob ) - Soonchunhyang University College of Medicine Soonchunhyang University Hospital Department of Urology
김제종 ( Kim Je-Jong ) - Korea University College of Medicine Department of Urology
문두건 ( Moon Du-Geon ) - Korea University College of Medicine Department of Urology

Abstract


Purpose: To validate a novel arteriogenic erectile dysfunction (ED) model with atherosclerosis (AS) based on molecular and histologic evidence induced by chronic pelvic ischemia (CPI) and determine effect of phosphodiesterase-5 inhibitor treatment.

Materials and Methods: Twenty 16-week-old male Sprague?Dawley rats were divided into three experimental groups (Group I, untreated sham-operated rats with regular diet; Group II, CPI with cholesterol diet; Group III, CPI model with cholesterol diet and mirodenafil). Erectile function was accessed using maximum intracavernous pressure (ICP) and ICP/mean arterial pressure (MAP). Molecular changes were examined by western blot analysis using hypoxia inducible factor 1-alpha (HIF-1α), endothelial nitric oxide synthase (eNOS), and transforming growth factor beta-1 (TGF-β1) antibodies. Collagen change was evaluated by Masson's trichrome staining.

Results: In vivo measurements of ICP and ICP/MAP in Group II were significantly lower than those in Group I (p<0.01). Smooth muscle/collagen ratio in Group II was significantly lower than that in Group I (p<0.05). After treatment with mirodenafil for four weeks, Group III showed significantly higher levels of ICP and ICP/MAP than Group II (p<0.05). Western blot analysis showed that HIF-1α and TGF-β1 levels were significantly higher in Group II whereas eNOS levels were significantly lower in Group II than those in Group I or III.

Conclusions: A novel arteriogenic ED with AS model is successfully induced by CPI and validated based on molecular and histologic evidences.

키워드

Atherosclerosis; Erectile dysfunction; Phosphodiesterase 5 Inhibitors; Vasculogenic impotence

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