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Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Inhibits Benign Prostatic Hyperplasia In Vitro and In Vivo

The World Journal of Men′s Health 2020년 38권 3호 p.385 ~ 396
Song Jun-Hui, 황병두, Chung Hyun-Joo, 문보경, 김진욱, 고기성, Kim Bae-Whan, Kim Won-Ryong, 김원재, 명순철, 문성권,
소속 상세정보
 ( Song Jun-Hui ) - Chung-Ang University College of Biotechnology and Natural Resources Department of Food and Nutrition
황병두 ( Hwang Byung-Doo ) - Chung-Ang University College of Biotechnology and Natural Resources Department of Food and Nutrition
 ( Chung Hyun-Joo ) - Chung-Ang University College of Medicine Department of Urology
문보경 ( Moon Bo-Kyung ) - Chung-Ang University College of Biotechnology and Natural Resources Department of Food and Nutrition
김진욱 ( Kim Jin-Wook ) - Chung-Ang University College of Medicine Department of Urology
고기성 ( Ko Ki-Sung ) - Chung-Ang University College of Medicine Department of Medicine
 ( Kim Bae-Whan ) - Resvera Co. Ltd.
 ( Kim Won-Ryong ) - Resvera Co. Ltd.
김원재 ( Kim Wun-Jae ) - Chungbuk National University College of Medicine Department of Urology
명순철 ( Myung Soon-Chul ) - Chung-Ang University College of Medicine Department of Urology
문성권 ( Moon Sung-Kwon ) - Chung-Ang University College of Biotechnology and Natural Resources Department of Food and Nutrition

Abstract


Purpose: In this study, we tested whether the resveratrol-enriched peanut sprout extracts cultivated with fermented sawdust medium (PSEFS) could suppress benign prostatic hyperplasia (BPH) in vitro and in vivo.

Materials and Methods: The mode of action of PSEFS was estimated by employing high-performance liquid chromatography analysis, MTT assay, cell counting, cell cycle analysis, immunoblots, and immunoprecipitation and electrophoretic mobility shift assay. In vivo efficacy of PSEFS was analyzed in BPH animal model via immunostaining and enzyme-linked immunosorbent assay.

Results: We selected the Yesan peanut sprout variety, which contains the highest level of resveratrol. The resveratrol levels in PSEFS were higher than those obtained with hydroponic technology. PSEFS treatment induced cell cycle arrest at the G1-phase by downregulating CDK4 and cyclin D1 via p21WAF1 induction in the RWPE-1 and WPMY prostate cells, thereby decreasing their proliferation. Treatment with PSEFS decreased ERK1/2 phosphorylation and increased JNK phosphorylation. The levels of DNA-bound transcription factors associated with proliferation (nuclear factor-κB, Sp-1, and AP-1) decreased upon PSEFS treatment in both prostate cells. Additionally, the levels of the molecular markers of BPH development (5α-reductase, androgen receptor, fibroblast growth factor, Bcl-2, and Bax) also changed by the addition of PSEFS. Finally, in a testosterone propionate-induced BPH model in rats, PSEFS administration attenuated the size, weight, and thickness of prostate tissues with no signs of death.

Conclusions: These results showed that PSEFS inhibited BPH both in vitro and in vivo and might be useful in the development of a potential BPH therapy.

키워드

Benign prostatic hyperplasia; Cell cycle; Peanut; Resveratrol; RWPE-1; Sawdust

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