잠시만 기다려 주세요. 로딩중입니다.

Liquid biopsy using extracellular vesicle-derived DNA in lung adenocarcinoma

Journal of Pathology and Translational Medicine 2020년 54권 6호 p.453 ~ 461
김인애, 허재영, 김희정, 이승은, 김완섭, 이계영,
소속 상세정보
김인애 ( Kim In-Ae ) - Konkuk University School of Medicine Department of Internal Medicine
허재영 ( Hur Jae-Young ) - Konkuk University School of Medicine Department of Pathology
김희정 ( Kim Hee-Joung ) - Konkuk University School of Medicine Department of Internal Medicine
이승은 ( Lee Seung-Eun ) - Konkuk University School of Medicine Department of Pathology
김완섭 ( Kim Wan-Seop ) - Konkuk University School of Medicine Department of Pathology
이계영 ( Lee Kye-Young ) - Konkuk University School of Medicine Department of Internal Medicine

Abstract


Blood liquid biopsy has emerged as a way of overcoming the clinical limitations of repeat biopsy by testing for the presence of acquired resistance mutations to therapeutic agents. Despite its merits of repeatability and non-invasiveness, this method is currently only used as a supplemental test due to a relatively low sensitivity rate of 50%?60%, and cannot replace tissue biopsy. The circulating tumor DNAs used in blood liquid biopsies are passive products of fragmented DNA with a short half-life released following tumor cell death; the low sensitivity seen with liquid blood biopsy results from this instability, which makes increasing the sensitivity of this test fundamentally difficult. Extracellular vesicles (EVs) are ideal carriers of cancer biomarkers, as cancer cells secret an abundance of EVs, and the contents of tumor cell-originated EVs reflect the molecular and genetic composition of parental cells. In addition, EV-derived DNAs (EV DNAs) consist of large-sized genomic DNAs and tumor-specific oncogenic mutant DNAs. For these reasons, liquid biopsy using EV DNA has the potential to overcome issues arising from tissue shortages associated with small biopsies, which are often seen in lung cancer patients, and the biopsy product can be used in other diagnostic methods, such as epidermal growth factor receptor (EGFR) mutation testing and next-generation sequencing (NGS). A higher sensitivity can be achieved when EV DNAs obtained from bronchoalveolar lavage fluid (BALF) are used rather than those from blood. BALF, when obtained close to the tumor site, is a promising liquid biopsy tool, as it enables the gathering of both cellular and non-cellular fractions of the tumor microenvironment, and provides increased diagnostic sensitivity when compared to blood.

키워드

Lung adenocarcinoma; EV-based EGFR genotyping; Liquid biopsy; Extracellular vesicles; EV-derived DNA

원문 및 링크아웃 정보

등재저널 정보