잠시만 기다려 주세요. 로딩중입니다.

Can BAP1 expression loss in mesothelial cells be an indicator of malignancy?

Journal of Pathology and Translational Medicine 2020년 54권 6호 p.497 ~ 503
Ozdemir Hanife Gulnihal, Kokten Sermin Coban, Barisik Nagehan Ozdemir,
소속 상세정보
 ( Ozdemir Hanife Gulnihal ) - Osmaniye Public Hospital Department of Pathology
 ( Kokten Sermin Coban ) - University of Health Sciences Kartal Dr Lutfi Kirdar Training and Research Hospital
 ( Barisik Nagehan Ozdemir ) - University of Health Sciences Kartal Dr Lutfi Kirdar Training and Research Hospital

Abstract


Background: Malignant mesothelioma is a highly aggressive tumor that can be confused with a benign mesothelial lesion, especially cytomorphologic lesions. BRCA1-associated protein 1 (BAP1) acts as a tumor suppressor. In this study, we aim to investigate the value of BAP1 staining of malignant mesothelioma cases with expression loss and diagnosis in cell block and biopsy tissue.

Methods: Between January 2009 and March 2017, 64 mesotheliomas, 117 reactive mesothelial hyperplasias, and 20 fibrinous pleuritis/pericarditis were diagnosed with morphologic and immunohistochemical findings in our pathology clinic and were included in the study. Formalin-fixed, paraffin-embedded tissues were immunohistochemically examined for BAP1. Inflammatory and stromal cells were used as positive internal controls. BAP1 was assessed for nuclear staining in mesothelial cells.

Results: Examinations of the relationship between patient diagnosis and BAP1 biopsy status showed that the BAP1 loss rate (76.6%) was significantly higher in malignant mesothelioma cases than in other benign diseases (0%) (p<.001). Sensitivity and specificity were 76.56% and 100%, respectively, for biopsy tissue from malignant mesothelioma. Sensitivity and specificity were both 100% for BAP1 test on cell block tissue. Furthermore, the consistency between BAP1 cell block and biopsy results was excellent (ĸ=0.90) and the correlation was significant (p<.001).

Conclusions: This study shows that BAP1 expression loss in both cytology and biopsy tissue in biopsy-confirmed malignant mesothelioma cases is an essential parameter for malignant mesothelioma diagnosis.

키워드

Asbestosis; BAP1; Immunohistochemistry; Malignant mesothelioma; Pleura

원문 및 링크아웃 정보

등재저널 정보