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Effects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model

Tuberculosis and Respiratory Diseases 2021년 84권 1호 p.55 ~ 66
한희재, 오은이, 이재현, 박중원, 박혜정,
소속 상세정보
한희재 ( Han Hee-Jae ) - Gangnam Severance Hospital Department of Internal Medicine
오은이 ( Oh Eun-Yi ) - Yonsei University College of Medicine Institute of Allergy
이재현 ( Lee Jae-Hyun ) - Yonsei University College of Medicine Department of Internal Medicine
박중원 ( Park Jung-Won ) - Yonsei University College of Medicine Department of Internal Medicine
박혜정 ( Park Hye-Jung ) - Gangnam Severance Hospital Department of Internal Medicine

Abstract


Background: Particulate matter 10 (PM10; airborne particles <10 μm) inhalation has been demonstrated to induce airway and lung diseases. In this study, we investigate the effects of PM10 inhalation on RNA expression in lung tissues using a murine model.

Methods: Female BALB/c mice were affected with PM10, ovalbumin (OVA), or both OVA and PM10. PM10 was administered intranasally while OVA was both intraperitoneally injected and intranasally administered. Treatments occurred 4 times over a 2-week period. Two days after the final challenges, mice were sacrificed. Full RNA sequencing using lung homogenates was conducted.

Results: While PM10 did not induce cell proliferation in bronchoalveolar fluid or lead to airway hyper-responsiveness, it did cause airway inflammation and lung fibrosis. Levels of interleukin 1β, tumor necrosis factor-α, and transforming growth factor-β in lung homogenates were significantly elevated in the PM10-treated group, compared to the control group. The PM10 group also showed increased RNA expression of Rn45a, Snord22, Atp6v0c-ps2, Snora28, Snord15b, Snora70, and Mmp12. Generally, genes associated with RNA splicing, DNA repair, the inflammatory response, the immune response, cell death, and apoptotic processes were highly expressed in the PM10-treated group. The OVA/PM10 treatment did not produce greater effects than OVA alone. However, the OVA/PM10-treated group did show increased RNA expression of Clca1, Snord22, Retnla, Prg2, Tff2, Atp6v0c-ps2, and Fcgbp when compared to the control groups. These genes are associated with RNA splicing, DNA repair, the inflammatory response, and the immune response.

Conclusion: Inhalation of PM10 extensively altered RNA expression while also inducing cellular inflammation, fibrosis, and increased inflammatory cytokines in this murine mouse model.

키워드

Particulate Matter; RNA Sequencing; Lung

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